Source:http://linkedlifedata.com/resource/pubmed/id/18266910
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
2008-4-14
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pubmed:abstractText |
The centrosome-nucleus attachment is a prerequisite for faithful chromosome segregation during mitosis. We addressed the function of the nuclear envelope (NE) protein Sun-1 in centrosome-nucleus connection and the maintenance of genome stability in Dictyostelium discoideum. We provide evidence that Sun-1 requires direct chromatin binding for its inner nuclear membrane targeting. Truncation of the cryptic N-terminal chromatin-binding domain of Sun-1 induces dramatic separation of the inner from the outer nuclear membrane and deformations in nuclear morphology, which are also observed using a Sun-1 RNAi construct. Thus, chromatin binding of Sun-1 defines the integrity of the nuclear architecture. In addition to its role as a NE scaffold, we find that abrogation of the chromatin binding of Sun-1 dissociates the centrosome-nucleus connection, demonstrating that Sun-1 provides an essential link between the chromatin and the centrosome. Moreover, loss of the centrosome-nucleus connection causes severe centrosome hyperamplification and defective spindle formation, which enhances aneuploidy and cell death significantly. We highlight an important new aspect for Sun-1 in coupling the centrosome and nuclear division during mitosis to ensure faithful chromosome segregation.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chromatin,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protozoan Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1600-0854
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
708-24
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pubmed:meshHeading |
pubmed-meshheading:18266910-Amino Acid Sequence,
pubmed-meshheading:18266910-Animals,
pubmed-meshheading:18266910-Centrosome,
pubmed-meshheading:18266910-Chromatin,
pubmed-meshheading:18266910-DNA-Binding Proteins,
pubmed-meshheading:18266910-Dictyostelium,
pubmed-meshheading:18266910-Genomic Instability,
pubmed-meshheading:18266910-Molecular Sequence Data,
pubmed-meshheading:18266910-Nuclear Envelope,
pubmed-meshheading:18266910-Nuclear Proteins,
pubmed-meshheading:18266910-Protein Structure, Quaternary,
pubmed-meshheading:18266910-Protozoan Proteins,
pubmed-meshheading:18266910-RNA Interference,
pubmed-meshheading:18266910-Recombinant Fusion Proteins
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pubmed:year |
2008
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pubmed:articleTitle |
Dictyostelium Sun-1 connects the centrosome to chromatin and ensures genome stability.
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pubmed:affiliation |
Center for Biochemistry I, Medical Faculty, University of Cologne, Joseph-Stelzmann-Strasse 52, 50931 Cologne, Germany.
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pubmed:publicationType |
Journal Article
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