Linked Life Data

18265374

Source:http://linkedlifedata.com/resource/pubmed/id/18265374

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-2-11
pubmed:abstractText
Extracts prepared from the isolated nuclei of cultured cells have been instrumental in dissecting the mechanisms by which transcription and mRNA processing occur. These extracts are able to recapitulate accurate transcription initiation and splicing in vitro, which has been useful in direct functional studies. They also serve as the starting material for purification of proteins that can then be reassembled in functional studies or examined in more detail biochemically. This unit describes the preparation of nuclear extracts from cultured cells and optimized production of transcriptionally active extracts from HeLa cells. Additional protocols describe optimization of the method to increase the yield of specific proteins, adaptation of the method for downstream applications such as affinity purification, and preparation of the cytoplasmic (S-100) fraction.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1934-3647
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
Chapter 12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 12.1
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Preparation of nuclear and cytoplasmic extracts from mammalian cells.
pubmed:affiliation
The Stowers Institute for Medical Research, Kansas City, Missouri, USA.
pubmed:publicationType
Journal Article