Linked Life Data

18265335

Source:http://linkedlifedata.com/resource/pubmed/id/18265335

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-2-11
pubmed:abstractText
Analysis of gene function frequently requires the formation of mammalian cell lines that contain the studied gene in a stably integrated form. Approximately one in 10(4) cells in a transfection will stably integrate DNA (the efficiency can vary depending on the cell type). Therefore, a dominant, selectable marker is used to permit isolation of stable transfectants. In the first part of this unit, the procedure for determining selection conditions and the resulting stable transfection is presented and the most commonly used selectable markers are discussed. The second protocol includes conditions for thirteen markers commonly used for selection of mammalian cells. A third protocols describes selection of transfected cells from the total population soon after transfection with plasmids that express both the gene of interest and a selection tag. Optimization of transfection conditions can be facilitated by a simple staining assay detailed in a .
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1934-3647
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
Chapter 9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 9.5
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Selection of transfected mammalian cells.
pubmed:affiliation
Brigham and Women's Hospital, Boston, Massachusetts, USA.
pubmed:publicationType
Journal Article