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PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-2-11
pubmed:abstractText
The uses of monoclonal antibodies as enzyme conjugates and as immunoaffinity reagents require their purification from the crude ascites fluid. This unit provides protocols for purification using protein A-Sepharose chromatography and affinity chromatography, which are superior to ammonium sulfate precipitation, gel filtration, or ion-exchange chromatography for the preparation of contaminant-free antibody.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1934-3647
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
Chapter 11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit11.11
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Purification of monoclonal antibodies.
pubmed:affiliation
Allelix Inc., Mississauga, Ontario.
pubmed:publicationType
Journal Article