Source:http://linkedlifedata.com/resource/pubmed/id/18265041
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:dateCreated |
2008-2-11
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pubmed:abstractText |
DNA extracted from lambda-derived vectors is typically subcloned into plasmid or filamentous phage vectors. The first two protocols describe methods for isolating phage DNA from large- and medium-scale liquid lysates. These two methods use either density-gradient centrifugation or ion-exchange chromatography to purify the phage particles. The third protocol describes a rapid procedure for isolating phage DNA, suitable for small-scale liquid lysates.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1934-3647
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
Chapter 1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
Unit1.13
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pubmed:meshHeading | |
pubmed:year |
2001
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pubmed:articleTitle |
Preparing lambda DNA from phage lysates.
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pubmed:affiliation |
Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
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pubmed:publicationType |
Journal Article
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