Linked Life Data

182564

Source:http://linkedlifedata.com/resource/pubmed/id/182564

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1976-10-29
pubmed:abstractText
The copper-IUD has been proved to be more effective for contraception than the popular IUD. To understand this mechanism of action, the effects of metallic ions on estrogen and progesterone receptors were examined in the cytosol of estrogen primed with female rabbits. The 274,200 X G supernatant of the uterine homogenate was used as the cytosol. The cytosol and 3H-progesterone (3.12 X 10(-9)M) or 3H-estradiol-17 beta (3.46 X 10(-9)M), with or without various metallic ions of different concentrations had been incubated for 2 hours. Bindings of steroids were estimated by the dextran coated charcoal assay (0.001% dextran and 0.1% Norite A) and were evaluated by 5 approximately 20% sucrose linear gradient centrifugation 1) Effect of various metallic ions on steroid hormone-receptor binding (determined by dextran coated charcoal assay. Fig. 1) : Thesteroid hormone-receptor interactions were markedly inhibited by Cu++, Fe++, and Zn++ ions and moderately by Mn++, but K+ and Ca++ increased of slightly affected the binding at concentrations between 10(-2)M and 10(-4)M. There were some differences between estrogen and progesterone receptors in their sensitivities to various metallic ions. 2) The effect of copper ion on the binding of steroid to the receptor : The dextran coated charcoal assay (Fig. 2) demonstrated that the inhibitory effect of Cu++ appeared at 10(-6)M and the steroidhormone-receptor bound decreased down to 10% at 10(-2)M Cu++. The estrogen receptor was less affected by copper than the progesterone receptor. It was demonstrated by 5 approximately 20% sucrose linear gradient centrifugation (Fig 3) that estrogen and progesterone receptors, which both sedimented at 8 S, were changed to more sedimenting forms in the presence of 10(-4)M Cu++, and were dissociated to 6.5 S form with moderate loss of steriod hormone binding affinity in 10(-2)M Cu++. The kinetic study (Fig 4), determined by dextran coated charcoal, showed that Cu++ was a competitive inhibitor against steroid hormone receptor bindings with Ki in equilibrium 2.7 X 10(-5)M to estrogen receptor (Kd in equilibrium 1.4 X 10(-9)M), and with Ki in equilibrium 5.1 X 10(-6M to progesterone receptor (Kd in equilibrium 8.1 X 10(-10)M). These results indicate the inhibiting factor of copper is the direct interference at the steriod binding site of the receptor, resulting in the increase in the effectiveness of the IUD. While progesterone receptor is more affected by copper ion than estrogen receptor, it is suggested that estrogen receptor survives longer than progesterone receptor and thus the biolgoical effect of copper seems to be somewhat estrogenic.
pubmed:language
jpn
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0029-0661
pubmed:author
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
51
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
856-63
pubmed:dateRevised
2011-7-27
pubmed:meshHeading
pubmed:year
1975
pubmed:articleTitle
[The effect of metallic ions on steroid hormone receptors in the rabbit uterus (author's transl)].
pubmed:publicationType
Journal Article, English Abstract