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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0025663,
umls-concept:C0030415,
umls-concept:C0035668,
umls-concept:C0184511,
umls-concept:C0185117,
umls-concept:C0231441,
umls-concept:C0332306,
umls-concept:C0443218,
umls-concept:C0520510,
umls-concept:C0936012,
umls-concept:C0949307,
umls-concept:C1707903,
umls-concept:C2349975,
umls-concept:C2827483,
umls-concept:C2911684
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pubmed:dateCreated |
2008-3-3
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pubmed:abstractText |
Archival formalin-fixed paraffin-embedded (FFPE) tissues represent an abundant source of clinical specimens; however their use is limited in applications involving analysis of gene expression due to RNA degradation and modification during fixation and processing. This study improved the quality of RNA extracted from FFPE by introducing a heating step into the selected extraction protocols. Further, it evaluated a novel pre-amplification system (PreAmp) designed to enhance expression analysis from tissue samples using assays with a range of amplicon size (62-164 bp).
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-10365909,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-10398146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-10536153,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-10951397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-11159180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-11272893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-11568892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-11786092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-11846610,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-12218216,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-12270266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-12462400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-12466110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-12552078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-12763523,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-12782023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-13905167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-14695316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-15951835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-16116034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-16171460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-16469371,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-16517976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-16864893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-16900335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-16932070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-17156473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-17603869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-17660800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-7459328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-7731989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-8908518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18254955-8908519
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1472-6750
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
2008
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pubmed:articleTitle |
Improved RNA quality and TaqMan Pre-amplification method (PreAmp) to enhance expression analysis from formalin fixed paraffin embedded (FFPE) materials.
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pubmed:affiliation |
Deptment of Histopathology, University of Dublin, Trinity College, Dublin, Ireland. jinghual@tcd.ie
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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