Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1991-3-15
pubmed:abstractText
Monoclonal antibodies were raised against a bacterial fusion protein containing amino acids 364 to 623 of the major nonstructural protein, NS-1, of minute virus of mice (MVMp), an autonomous parvovirus. By immunoblot analyses, these antibodies all recognized an 83-kDa protein in MVM-infected mouse fibroblast cells. Indirect immunofluorescence studies showed that five of the six react against a nuclear protein in MVM-infected mouse cells resulting in discrete foci of fluorescence. These foci do not correspond with the nucleoli, the site of MVM DNA replication. The epitopes of the antibodies were mapped using carboxy-terminal deleted bacterial fusion proteins derived from the plasmid encoding the original antigen and showed that four distinct epitopes were recognized by the different antibodies. A 25-amino-acid peptide was used in competition ELISAs to confirm the location of the epitope recognized by two antibodies CE10 and AC6. Preliminary characterization of an NS-1/NS-2 fusion protein synthesized in insect cells using a baculovirus expression vector showed that this fusion protein is also localized within the nucleus; however, in contrast, the full-length NS-1 polypeptide is located within the cytoplasm.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0042-6822
pubmed:author
pubmed:issnType
Print
pubmed:volume
181
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
35-45
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:1825254-Amino Acid Sequence, pubmed-meshheading:1825254-Animals, pubmed-meshheading:1825254-Antibodies, Monoclonal, pubmed-meshheading:1825254-Base Sequence, pubmed-meshheading:1825254-Capsid, pubmed-meshheading:1825254-Chromosome Deletion, pubmed-meshheading:1825254-Fluorescent Antibody Technique, pubmed-meshheading:1825254-Genes, Viral, pubmed-meshheading:1825254-Mice, pubmed-meshheading:1825254-Minute virus of mice, pubmed-meshheading:1825254-Molecular Sequence Data, pubmed-meshheading:1825254-Nuclear Proteins, pubmed-meshheading:1825254-Oligonucleotide Probes, pubmed-meshheading:1825254-Plasmids, pubmed-meshheading:1825254-Recombinant Fusion Proteins, pubmed-meshheading:1825254-Restriction Mapping, pubmed-meshheading:1825254-Viral Core Proteins, pubmed-meshheading:1825254-Viral Nonstructural Proteins
pubmed:year
1991
pubmed:articleTitle
Monoclonal antibodies to the major nonstructural nuclear protein of minute virus of mice.
pubmed:affiliation
Department of Biochemistry, University of British Columbia, Vancouver, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't