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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1991-2-28
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pubmed:databankReference |
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pubmed:abstractText |
A heparin-binding mitogen was isolated from conditioned medium of human embryonic lung fibroblasts. It exhibited broad target-cell specificity whose pattern was distinct from that of any known growth factor. It rapidly stimulated tyrosine phosphorylation of a 145-kDa protein in responsive cells, suggesting that its signaling pathways involved activation of a tyrosine kinase. Purification identified a major polypeptide with an apparent molecular mass of 87 kDa under reducing conditions. Partial amino acid sequence analysis and cDNA cloning revealed that it was a variant of hepatocyte growth factor, a mitogen thought to be specific for hepatic cells and structurally related to plasminogen. Recombinant expression of the cDNA in COS-1 cells established that it encoded the purified growth factor. Its site of synthesis and spectrum of targets imply that this growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-2137248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-2139229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-2142596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-2142751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-2165484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-2180699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-2473389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-2524251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-2528952,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-2531289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-2531695,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-2692563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-2821619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-2915979,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-2930999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-2992362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-2999980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-3002607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-3027025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-3052279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-3143380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-3276728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-3304142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-3318882,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-3319692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-3511384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-3525608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-3569635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-4226004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-6089648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-6260373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-6300662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-6314450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-6373375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1824873-7309790
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
88
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pubmed:owner |
NLM
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pubmed:authorsComplete |
N
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pubmed:pagination |
415-9
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:1824873-Amino Acid Sequence,
pubmed-meshheading:1824873-Animals,
pubmed-meshheading:1824873-Cell Line,
pubmed-meshheading:1824873-Cloning, Molecular,
pubmed-meshheading:1824873-DNA,
pubmed-meshheading:1824873-DNA Replication,
pubmed-meshheading:1824873-Enzyme Activation,
pubmed-meshheading:1824873-Fibroblasts,
pubmed-meshheading:1824873-Genetic Variation,
pubmed-meshheading:1824873-Growth Substances,
pubmed-meshheading:1824873-Hepatocyte Growth Factor,
pubmed-meshheading:1824873-Humans,
pubmed-meshheading:1824873-Kinetics,
pubmed-meshheading:1824873-Lung,
pubmed-meshheading:1824873-Mice,
pubmed-meshheading:1824873-Mice, Inbred Strains,
pubmed-meshheading:1824873-Mitogens,
pubmed-meshheading:1824873-Molecular Sequence Data,
pubmed-meshheading:1824873-Oligonucleotide Probes,
pubmed-meshheading:1824873-Protein-Tyrosine Kinases,
pubmed-meshheading:1824873-Recombinant Proteins,
pubmed-meshheading:1824873-Restriction Mapping,
pubmed-meshheading:1824873-Transfection
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pubmed:year |
1991
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pubmed:articleTitle |
A broad-spectrum human lung fibroblast-derived mitogen is a variant of hepatocyte growth factor.
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