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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2008-4-4
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pubmed:abstractText |
The Red recombinase system of bacteriophage lambda has been used to inactivate chromosomal genes in various bacteria and fungi. The procedure consists of electroporating a polymerase chain reaction (PCR) fragment that was obtained with a 1- or 3-step PCR protocol and that carries an antibiotic cassette flanked by a region homologous to the target locus into a strain that expresses the lambda Red recombination system.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18248677-10829079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18248677-11071951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18248677-11724939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18248677-12791131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18248677-12898223,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18248677-13129645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18248677-14617778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18248677-14739337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18248677-15165237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18248677-15686549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18248677-15731059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18248677-15987659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18248677-16477005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18248677-16763151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18248677-17337571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18248677-1899844,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18248677-3340535,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18248677-4923748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18248677-8904338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18248677-9555887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18248677-9661666
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1471-2199
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
20
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pubmed:dateRevised |
2011-9-26
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pubmed:meshHeading |
pubmed-meshheading:18248677-Bacteriophage lambda,
pubmed-meshheading:18248677-Blotting, Southern,
pubmed-meshheading:18248677-DNA, Bacterial,
pubmed-meshheading:18248677-Electroporation,
pubmed-meshheading:18248677-Genetic Engineering,
pubmed-meshheading:18248677-Mutagenesis,
pubmed-meshheading:18248677-Mutation,
pubmed-meshheading:18248677-Plasmids,
pubmed-meshheading:18248677-Polymerase Chain Reaction,
pubmed-meshheading:18248677-Pseudomonas aeruginosa,
pubmed-meshheading:18248677-Pyocyanine,
pubmed-meshheading:18248677-Recombinases,
pubmed-meshheading:18248677-Reproducibility of Results,
pubmed-meshheading:18248677-Sequence Deletion
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pubmed:year |
2008
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pubmed:articleTitle |
Use of the lambda Red recombinase system to rapidly generate mutants in Pseudomonas aeruginosa.
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pubmed:affiliation |
Department of Surgery, Harvard Medical School and Massachusetts General Hospital, Boston, MA 02114, USA. lesic@molbio.mgh.harvard.edu
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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