Source:http://linkedlifedata.com/resource/pubmed/id/18246604
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2008-2-1
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| pubmed:abstractText |
Clinical studies indicate potential differences in the efficacy of immunoglobulin (Ig) preparations in patients with sepsis. A recent meta-analysis showed improved survival rates with IgM-enriched Igs. It was the objective of the present study to characterize microcirculatory actions of different clinically used Ig preparations in a rodent endotoxin model by intravital microscopy. Male Syrian golden hamsters 6 to 8 weeks old with a body weight of 60 to 80 g were investigated by intravital fluorescence microscopy. Endotoxemia was induced by administration of 2 mg/kg (i.v.) endotoxin (LPS, Escherichia coli). Two different Ig preparations containing IgM, IgA, and IgG (intravenous IgM group; n = 6; 5 mL Pentaglobin/kg body weight, i.v.) or exclusively IgG (intravenous IgG group; n = 5; 5 mL Flebogamma/kg body weight, i.v.) were applied 5 min before LPS. Saline-treated endotoxemic animals served as controls (control; n = 8). In controls, LPS induced massive leukocyte-endothelial cell interactions, pronounced microvascular leakage, a decrease of systemic platelet count, and distinct capillary perfusion failure (P < 0.05). Both intravenous IgM and IgG reduced venular leakage (P< 0.05) and ameliorated the decrease in platelet count (P < 0.05). Of interest, intravenous IgM was capable of significantly (P< 0.05) reducing leukocyte adhesion in venules. This was associated with normalization of capillary perfusion at 24 h of endotoxemia, whereas intravenous IgG could not prevent LPS-mediated microvascular perfusion failure. We demonstrate that IgM-enriched Igs are superior to IgG alone in attenuating LPS-induced leukocytic inflammation and microcirculatory dysfunction. Our findings can explain better efficacy of IgM-enriched Igs in patients with severe sepsis.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
1073-2322
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
29
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
133-9
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| pubmed:meshHeading |
pubmed-meshheading:18246604-Animals,
pubmed-meshheading:18246604-Cell Adhesion,
pubmed-meshheading:18246604-Cricetinae,
pubmed-meshheading:18246604-Disease Models, Animal,
pubmed-meshheading:18246604-Endothelial Cells,
pubmed-meshheading:18246604-Endotoxemia,
pubmed-meshheading:18246604-Humans,
pubmed-meshheading:18246604-Immunoglobulin G,
pubmed-meshheading:18246604-Immunoglobulin M,
pubmed-meshheading:18246604-Immunoglobulins, Intravenous,
pubmed-meshheading:18246604-Leukocyte Rolling,
pubmed-meshheading:18246604-Leukocytes,
pubmed-meshheading:18246604-Lipopolysaccharides,
pubmed-meshheading:18246604-Male,
pubmed-meshheading:18246604-Mesocricetus,
pubmed-meshheading:18246604-Microcirculation,
pubmed-meshheading:18246604-Microscopy, Fluorescence,
pubmed-meshheading:18246604-Sepsis
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| pubmed:year |
2008
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| pubmed:articleTitle |
Immunoglobulin M-enriched human intravenous immunoglobulins reduce leukocyte-endothelial cell interactions and attenuate microvascular perfusion failure in normotensive endotoxemia.
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| pubmed:affiliation |
Department of Surgery, Klinikum Grosshadern, Ludwig-Maximilians-University, Munich, Germany. Johannes.Hoffman@med.uni-muenchen.de
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| pubmed:publicationType |
Journal Article
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