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rdf:type |
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lifeskim:mentions |
umls-concept:C0018042,
umls-concept:C0021467,
umls-concept:C0021469,
umls-concept:C0031667,
umls-concept:C0065042,
umls-concept:C0208973,
umls-concept:C0379710,
umls-concept:C0521449,
umls-concept:C0699789,
umls-concept:C1517892,
umls-concept:C1704666
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pubmed:issue |
15
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pubmed:dateCreated |
2008-4-8
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pubmed:abstractText |
The molecular mechanisms regulating the exit of caveolin from the Golgi complex are not fully understood. Cholesterol and sphingolipid availability affects Golgi vesiculation events and involves the activity of cytoplasmic phospholipase A(2) (cPLA(2)). We recently demonstrated that high expression levels of annexin A6 (AnxA6) perturb the intracellular distribution of cellular cholesterol, thereby inhibiting caveolin export from the Golgi complex. In the present study we show that in Chinese hamster ovary cells overexpressing AnxA6, sequestration of cholesterol in late endosomes, leading to reduced amounts of cholesterol in the Golgi, inhibits cPLA(2) activity and its association with the Golgi complex. This correlates with the blockage of caveolin export from the Golgi in cells treated with methyl arachidonyl fluorophosphonate, a Ca(2+)-dependent cPLA(2) inhibitor. AnxA6-mediated down-regulation of cPLA(2) activity was overcome upon the addition of exogenous cholesterol or transfection with small interfering RNA targeting AnxA6. These findings indicate that AnxA6 interferes with caveolin transport through the inhibition of cPLA(2).
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Annexin A6,
http://linkedlifedata.com/resource/pubmed/chemical/Arachidonic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Caveolin 1,
http://linkedlifedata.com/resource/pubmed/chemical/Cholesterol,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipases A2, Cytosolic,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphonic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/Sphingolipids,
http://linkedlifedata.com/resource/pubmed/chemical/methyl arachidonylfluorophosphonate
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
283
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10174-83
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pubmed:dateRevised |
2010-10-19
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pubmed:meshHeading |
pubmed-meshheading:18245088-Animals,
pubmed-meshheading:18245088-Annexin A6,
pubmed-meshheading:18245088-Arachidonic Acids,
pubmed-meshheading:18245088-CHO Cells,
pubmed-meshheading:18245088-COS Cells,
pubmed-meshheading:18245088-Caveolin 1,
pubmed-meshheading:18245088-Cercopithecus aethiops,
pubmed-meshheading:18245088-Cholesterol,
pubmed-meshheading:18245088-Cricetinae,
pubmed-meshheading:18245088-Cricetulus,
pubmed-meshheading:18245088-Enzyme Inhibitors,
pubmed-meshheading:18245088-Golgi Apparatus,
pubmed-meshheading:18245088-HeLa Cells,
pubmed-meshheading:18245088-Humans,
pubmed-meshheading:18245088-Phospholipases A2, Cytosolic,
pubmed-meshheading:18245088-Phosphonic Acids,
pubmed-meshheading:18245088-Protein Transport,
pubmed-meshheading:18245088-RNA, Small Interfering,
pubmed-meshheading:18245088-Sphingolipids
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pubmed:year |
2008
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pubmed:articleTitle |
Annexin A6-induced inhibition of cytoplasmic phospholipase A2 is linked to caveolin-1 export from the Golgi.
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pubmed:affiliation |
Departament de Biologia Cellular, Facultat de Medicina, Universitat de Barcelona, Barcelona, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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