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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1992-10-16
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pubmed:abstractText |
B-lymphocyte clones bearing an idiotypic Ig structure, which could also be found on the serum M-component, have been isolated from peripheral blood of a patient with monoclonal gammopathy (MG) using an immune-rosetting technique and limiting dilution. The majority of the cells had the surface phenotype id+/CD19+/CD20+/CD21+ or id+/CD19+/CD20+/CD21-. Some of the clones originated from spontaneous outgrowth of lymphocytes and some were derived from in vitro Epstein-Barr virus-infected lymphocytes. Three of the B-lymphocyte clones and the patient's bone marrow plasma cells appeared to have clonal identity as shown by the same Ig heavy chain gene rearrangements in Southern blot analysis using a JH probe. The study further supports the notion that the peripheral blood of patients with MG contains B lymphocytes that belong to the tumor clone.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0006-4971
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
78
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2642-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1824258-Antibodies, Monoclonal,
pubmed-meshheading:1824258-Antigens, CD,
pubmed-meshheading:1824258-B-Lymphocytes,
pubmed-meshheading:1824258-Blotting, Southern,
pubmed-meshheading:1824258-Cell Line,
pubmed-meshheading:1824258-Clone Cells,
pubmed-meshheading:1824258-DNA,
pubmed-meshheading:1824258-Female,
pubmed-meshheading:1824258-Fluorescent Antibody Technique,
pubmed-meshheading:1824258-Gene Rearrangement,
pubmed-meshheading:1824258-Genes, Immunoglobulin,
pubmed-meshheading:1824258-Humans,
pubmed-meshheading:1824258-Immunoglobulin Fab Fragments,
pubmed-meshheading:1824258-Immunoglobulin G,
pubmed-meshheading:1824258-Immunoglobulin Heavy Chains,
pubmed-meshheading:1824258-Immunoglobulin Idiotypes,
pubmed-meshheading:1824258-Isoelectric Focusing,
pubmed-meshheading:1824258-Middle Aged,
pubmed-meshheading:1824258-Paraproteinemias,
pubmed-meshheading:1824258-Phenotype,
pubmed-meshheading:1824258-Rosette Formation
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pubmed:year |
1991
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pubmed:articleTitle |
Establishment of idiotype bearing B-lymphocyte clones from a patient with monoclonal gammopathy.
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pubmed:affiliation |
Department of Oncology (Radiumhemmet), Karolinska Hospital, Stockholm, Sweden.
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pubmed:publicationType |
Journal Article,
Case Reports,
Research Support, Non-U.S. Gov't
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