Source:http://linkedlifedata.com/resource/pubmed/id/18228321
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
2008-1-29
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| pubmed:abstractText |
A basic property of myosin is its ability to interact with and translocate actin. This unit describes an in vitro motility assay that can be used to study the translocation, or sliding, of actin filaments by myosin bound to a coverslip. The assay makes use of the ability to image single F-actin filaments labeled with rhodamine phalloidin, a high-affinity fluorescent ligand using fluorescence microscopy. The system is fast, easy to set up and maintain, uses only small amounts of protein, and yields quantitative results.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
1934-2616
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
Chapter 13
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
Unit 13.2
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| pubmed:dateRevised |
2011-11-17
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| pubmed:meshHeading |
pubmed-meshheading:18228321-Actin Cytoskeleton,
pubmed-meshheading:18228321-Actins,
pubmed-meshheading:18228321-Animals,
pubmed-meshheading:18228321-Cell Migration Assays,
pubmed-meshheading:18228321-Cell Movement,
pubmed-meshheading:18228321-Humans,
pubmed-meshheading:18228321-Microscopy, Fluorescence,
pubmed-meshheading:18228321-Myosins,
pubmed-meshheading:18228321-Phalloidine,
pubmed-meshheading:18228321-Protein Transport,
pubmed-meshheading:18228321-Rhodamines,
pubmed-meshheading:18228321-Staining and Labeling
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| pubmed:year |
2001
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| pubmed:articleTitle |
In vitro motility assay to study translocation of actin by myosin.
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| pubmed:affiliation |
National Heart, Lung, and Blood Institute, Bethesda, Maryland, USA.
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| pubmed:publicationType |
Journal Article
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