Linked Life Data

18228225

Source:http://linkedlifedata.com/resource/pubmed/id/18228225

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2008-1-29
pubmed:databankReference
pubmed:abstractText
The Yarrowia lipolytica SRP72 gene product (YlSRP72), a homolog of the 72-kDa subunit of the mammalian SRP, encodes a putative protein of 602 amino acids. Northern blot analysis revealed a unique YlSRP72-specific transcript of 1.8 kb. The deduced amino acid sequence showed higher identities with the Srp72 proteins of euascomycetes than with hemiascomycetes. Chromosomal hybridization experiments showed that the YlSRP72 gene is located in chromosome V of the standard E150 strain of Y. lipolytica. Fluorescent microscopy revealed that the YlSRP72-GFP fusion protein was expressed in the cytoplasm and nucleus. The YlSRP72 gene was interrupted by the pop-out method; however, deletion of the gene proved to be lethal. This is in contrast to the results described for the Saccharomyces cerevisiae SRP72 gene, which is not essential for cell growth, and supports our previous finding with another component of the yeast recognition particle, YlSEC65. The present work suggests that SRP-dependent targeting is the main secretory pathway in Y. lipolytica, as has been described for higher eukaryotes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1139-6709
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
283-9
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Characterization of the Yarrowia lipolytica YlSRP72 gene, a component of the yeast signal recognition particle.
pubmed:affiliation
Department of Microbiology and Genetics, CIETUS, IMB/CSIC, University of Salamanca, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't