Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
1992-9-2
|
| pubmed:abstractText |
The presence of HBV-DNA sequences was evaluated in DNA extracted from serum samples, peripheral blood lymphocytes and liver biopsy specimens of five HBsAg/anti-HBe-positive carriers with chronic HDV infection. DNAs were tested by polymerase chain reaction (PCR) amplification technique using two pairs of oligonucleotide primers specific for the preC/C and S regions of the HBV; viral sequences were found exclusively in liver extracts and only in three out of the five cases. The direct sequencing of the amplified preC/C regions showed wild-type sequences in two cases, while in the third case a combination of 'wild' and 'e minus variant' viral populations was observed. Moreover, liver DNA of one positive case was electrophoresed through a low melting agarose gel and the following amplification, performed on DNA re-extracted from three different fragments of the gel, showed the presence of free HBV genomes but the absence of replicative intermediate forms. These results show that anti-HBe positivity is not constantly related to precore mutant HBV infection and suggest that HDV inhibits HBeAg production. Moreover, as it was observed in 'e minus' HBV variants, also during a chronic HBV wild-type infection, the viral replication might be suppressed to undetectable levels.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0168-8278
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
13 Suppl 4
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
S87-9
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:1822520-Base Sequence,
pubmed-meshheading:1822520-Carrier State,
pubmed-meshheading:1822520-DNA, Viral,
pubmed-meshheading:1822520-Genetic Variation,
pubmed-meshheading:1822520-Hepatitis B Antibodies,
pubmed-meshheading:1822520-Hepatitis B e Antigens,
pubmed-meshheading:1822520-Hepatitis B virus,
pubmed-meshheading:1822520-Hepatitis D,
pubmed-meshheading:1822520-Humans,
pubmed-meshheading:1822520-Liver,
pubmed-meshheading:1822520-Molecular Sequence Data,
pubmed-meshheading:1822520-Oligodeoxyribonucleotides,
pubmed-meshheading:1822520-Polymerase Chain Reaction
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
HBe antibody unrelated to 'e minus' hepatitis B virus variant infection in patients with chronic type D hepatitis.
|
| pubmed:affiliation |
Dipartimento di Medicina Interna, Università di Messina, Italy.
|
| pubmed:publicationType |
Journal Article
|