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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3-4
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pubmed:dateCreated |
1992-9-1
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pubmed:abstractText |
Zona-free zygotes, 2-cell, 4-cell, and 8-cell embryos, morulae, blastocysts and embryos exposed to biopsy and reconstruction were cryopreserved in microdrops of vitrification medium expelled directly into liquid nitrogen. While none of the zygotes and 2-cell embryos survived storage in liquid nitrogen, 54% 4-cell and 97% 8-cell embryos, 99% morulae, 88% blastocysts, 98% biopsied and 100% reconstructed embryos were intact one hour after thawing. Developmental potential of cryopreserved zona-free embryos evaluated after 30 h of in vitro culture was high. Of the frozen embryos, 49% 4-cell and 92% 8-cell embryos, 95% morulae, 79% blastocysts, 84% biopsied and 90% reconstructed embryos were capable of further cleavage. After the transfer of zona-free 8-cell embryos, morulae, blastocysts, biopsied and reconstructed embryos into day-1 recipients - 4 (16%), 14 (56%), 3 (12%), 0 and 8 (32%) live foetuses were recorded, respectively.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:issn |
0015-5500
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
37
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
171-8
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:1822444-Animals,
pubmed-meshheading:1822444-Cryopreservation,
pubmed-meshheading:1822444-Embryo, Mammalian,
pubmed-meshheading:1822444-Gastrula,
pubmed-meshheading:1822444-Mice,
pubmed-meshheading:1822444-Mice, Inbred Strains,
pubmed-meshheading:1822444-Morula,
pubmed-meshheading:1822444-Zona Pellucida,
pubmed-meshheading:1822444-Zygote
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pubmed:year |
1991
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pubmed:articleTitle |
Cryopreservation of mouse zona-free embryos and embryos exposed to biopsy and reconstruction by vitrification in microdrops.
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pubmed:affiliation |
Koltzov Institute of Developmental Biology of the Academy of Sciences of the USSR, Moscow.
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pubmed:publicationType |
Journal Article
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