Linked Life Data

18219447

Source:http://linkedlifedata.com/resource/pubmed/id/18219447

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2008-2-28
pubmed:abstractText
Tricyclic antidepressants and selective serotonin reuptake inhibitors are considered in theory to induce the outflow of neurotransmitters, norepinephrine, and serotonin from the synapses as a consequence of inhibiting their reuptake into the nerve terminals, resulting in the stimulation of glial cells surrounding the synapses in the brain. Then, we have investigated the direct actions of neurotransmitters on glial cell metabolism and function using rat C6 glioma cells as an in vitro model system and suggested that these neurotransmitters induce their differentiation probably through the production of 5alpha-reduced neurosteroids. On the other hand, the stimulation of the glioma cells with serotonin has been reported to enhance brain-derived neurotrophic factor (BDNF) gene expression, which may be closely related to the beneficial effects of antidepressant drugs. In the present study, to evaluate BDNF expression in differentiated glial cells, the glioma cells were pretreated with progesterone, and the effect of serotonin on BDNF messenger RNA levels in these cells was examined. Progesterone pretreatment enhanced the stimulatory action of serotonin on BDNF gene expression, and the enhancement of serotonin action observed in the cells pretreated with progesterone was almost completely abolished by finasteride, an inhibitor of the enzyme involved in the production of 5alpha-reduced neurosteroids. These findings propose the possibility that neurosteroid-mediated glial cell differentiation may result in the enhancement of serotonin-stimulated BDNF gene expression, which is considered to contribute to the survival, regeneration, and plasticity of neuronal cells in the brain, and hence, leading to the improvement of mood disorders and other symptoms in depressive patients.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0895-8696
pubmed:author
pubmed:issnType
Print
pubmed:volume
34
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
193-200
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:18219447-3-Oxo-5-alpha-Steroid 4-Dehydrogenase, pubmed-meshheading:18219447-Animals, pubmed-meshheading:18219447-Antidepressive Agents, Tricyclic, pubmed-meshheading:18219447-Brain, pubmed-meshheading:18219447-Brain-Derived Neurotrophic Factor, pubmed-meshheading:18219447-Cell Differentiation, pubmed-meshheading:18219447-Cell Line, Tumor, pubmed-meshheading:18219447-Drug Synergism, pubmed-meshheading:18219447-Enzyme Inhibitors, pubmed-meshheading:18219447-Finasteride, pubmed-meshheading:18219447-Gene Expression Regulation, pubmed-meshheading:18219447-Glioma, pubmed-meshheading:18219447-Neuroglia, pubmed-meshheading:18219447-Neuronal Plasticity, pubmed-meshheading:18219447-Presynaptic Terminals, pubmed-meshheading:18219447-Progesterone, pubmed-meshheading:18219447-RNA, Messenger, pubmed-meshheading:18219447-Rats, pubmed-meshheading:18219447-Serotonin, pubmed-meshheading:18219447-Serotonin Uptake Inhibitors, pubmed-meshheading:18219447-Up-Regulation
pubmed:year
2008
pubmed:articleTitle
Progesterone pretreatment enhances serotonin-stimulated BDNF gene expression in rat c6 glioma cells through production of 5alpha-reduced neurosteroids.
pubmed:affiliation
Department of Pharmacology, Tokushima University School of Medicine, 3-18-15 Kuramoto, Tokushima, 770-8503, Japan. km@basic.med.tokushima-u.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't