Source:http://linkedlifedata.com/resource/pubmed/id/18218958
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
Pt 2
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| pubmed:dateCreated |
2008-1-25
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| pubmed:databankReference | |
| pubmed:abstractText |
Four bacterial strains, designated K9(T), K105, K1012A and K101, were isolated from soil in Lithuania. All these strains could use n-butanol as a sole carbon source. The strains grew in a medium containing 12-120 mM n-butanol. The strains were strictly aerobic, Gram-positive endospore-formers. The best growth was achieved at 25 degrees C and pH 7.0 in medium containing 1 % (w/v) NaCl. The strains showed identical profiles of 16S-23S rRNA internal transcribed spacer PCR and nearly identical 16S rRNA gene PCR-RFLP electrophoretic patterns and physiological characteristics, demonstrating their relationship at the species level. The cellular fatty acid profile of K9(T) consisted of significant amounts of the C(15) branched-chain fatty acids iso-C(15 : 0) (16.78 %) and anteiso-C(15 : 0) (45.80 %). The diagnostic cell-wall diamino acid was meso-diaminopimelic acid. The 16S rRNA gene sequence of K9(T) showed the highest similarity to the sequences of Bacillus simplex DSM 1321(T) and Bacillus muralis LMG 20238(T) (98.3 and 97.7 %, respectively). The DNA G+C content was 37.4 mol%. Studies of DNA-DNA relatedness, morphological, physiological and chemotaxonomic analyses and phylogenetic data based on 16S rRNA gene sequencing allowed strains K9(T), K105, K1012A and K101 to be described as members of a novel species of the genus Bacillus, for which the name Bacillus butanolivorans sp. nov. is proposed. The type strain is K9(T) (=DSM 18926(T) =LMG 23974(T)).
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Butanols,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Ribosomal Spacer,
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acids,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Ribosomal, 16S,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Ribosomal, 23S
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| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
1466-5026
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
58
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
505-9
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| pubmed:meshHeading |
pubmed-meshheading:18218958-Bacillus,
pubmed-meshheading:18218958-Bacterial Typing Techniques,
pubmed-meshheading:18218958-Base Composition,
pubmed-meshheading:18218958-Butanols,
pubmed-meshheading:18218958-DNA, Bacterial,
pubmed-meshheading:18218958-DNA, Ribosomal Spacer,
pubmed-meshheading:18218958-Fatty Acids,
pubmed-meshheading:18218958-Genes, rRNA,
pubmed-meshheading:18218958-Industrial Microbiology,
pubmed-meshheading:18218958-Molecular Sequence Data,
pubmed-meshheading:18218958-Nucleic Acid Hybridization,
pubmed-meshheading:18218958-Polymerase Chain Reaction,
pubmed-meshheading:18218958-Polymorphism, Restriction Fragment Length,
pubmed-meshheading:18218958-RNA, Ribosomal, 16S,
pubmed-meshheading:18218958-RNA, Ribosomal, 23S,
pubmed-meshheading:18218958-Sequence Analysis, DNA,
pubmed-meshheading:18218958-Soil Microbiology,
pubmed-meshheading:18218958-Species Specificity
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| pubmed:year |
2008
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| pubmed:articleTitle |
Bacillus butanolivorans sp. nov., a species with industrial application for the remediation of n-butanol.
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| pubmed:affiliation |
Department of Plant Physiology and Microbiology, Vilnius University, Chiurlionio 21/27, Vilnius LT-03101, Lithuania. nomeda.kuisiene@gf.vu.lt
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| pubmed:publicationType |
Journal Article
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