|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2008-1-25
|
|
pubmed:abstractText |
Dental pulp stem cells (DPSCs) are a unique precursor population isolated from postnatal human dental pulp and have the ability to regenerate a reparative dentin-like complex. Canonical Wnt signaling plays a critical role in tooth development and stem cell self-renewal through beta-catenin. In this study, the regulation of odontoblast-like differentiation of DPSCs by canonical Wnt signaling was examined. DPSCs were stably transduced with canonical Wnt-1 or the active form of beta-catenin, with retrovirus-mediated infection. Northern blot analysis found that Wnt-1 strongly induced the expression of matricellular protein osteopontin, and modestly enhanced the expression of type I collagen in DPSCs. Unexpectedly, Wnt-1 inhibited alkaline phosphatase (ALP) activity and the formation of mineralized nodules in DPSCs. Moreover, over-expression of beta-catenin was also sufficient to suppress the differentiation and mineralization of DPSCs. In conclusion, our results suggest that canonical Wnt signaling negatively regulates the odontoblast-like differentiation of DPSCs.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18218837-11087820,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18218837-11149923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18218837-11346652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18218837-11719191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18218837-11980918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18218837-12015390,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18218837-12073157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18218837-12147742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18218837-12502739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18218837-14764716,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18218837-15121013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18218837-15486964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18218837-15649466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18218837-15802266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18218837-15829953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18218837-15866164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18218837-16043491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18218837-16223338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18218837-16499448,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18218837-16670761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18218837-17121988,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18218837-17351296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18218837-17518650,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18218837-8275945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18218837-9027589
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
D
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
0022-0345
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
87
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
126-30
|
|
pubmed:dateRevised |
2010-9-21
|
|
pubmed:meshHeading |
pubmed-meshheading:18218837-Alkaline Phosphatase,
pubmed-meshheading:18218837-Anthraquinones,
pubmed-meshheading:18218837-Blotting, Northern,
pubmed-meshheading:18218837-Blotting, Western,
pubmed-meshheading:18218837-Calcification, Physiologic,
pubmed-meshheading:18218837-Cell Differentiation,
pubmed-meshheading:18218837-Cells, Cultured,
pubmed-meshheading:18218837-Collagen Type I,
pubmed-meshheading:18218837-Coloring Agents,
pubmed-meshheading:18218837-Dental Pulp,
pubmed-meshheading:18218837-Genetic Vectors,
pubmed-meshheading:18218837-Humans,
pubmed-meshheading:18218837-Odontoblasts,
pubmed-meshheading:18218837-Osteopontin,
pubmed-meshheading:18218837-Polymerase Chain Reaction,
pubmed-meshheading:18218837-Retroviridae,
pubmed-meshheading:18218837-Signal Transduction,
pubmed-meshheading:18218837-Stem Cells,
pubmed-meshheading:18218837-Transduction, Genetic,
pubmed-meshheading:18218837-Wnt1 Protein,
pubmed-meshheading:18218837-beta Catenin
|
|
pubmed:year |
2008
|
|
pubmed:articleTitle |
Wnt/beta-catenin inhibits dental pulp stem cell differentiation.
|
|
pubmed:affiliation |
Laboratory of Molecular Signaling, Department of Biologic and Materials Sciences, School of Dentistry, The University of Michigan, Ann Arbor, MI 48109, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
|
