Linked Life Data

18217693

Source:http://linkedlifedata.com/resource/pubmed/id/18217693

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-1-25
pubmed:abstractText
An important step in cellular transformation and tumorigenesis is immortalization, in which cells gain the ability to grow indefinitely by bypassing cellular senescence that imposes a finite number of divisions in culture. Primary mouse embryonic fibroblast (MEF) cells have a limited growth capacity and on prolonged passaging spontaneously immortalize at a low frequency. In contrast to transformation of primary MEF cells that requires the presence of two cooperating oncogenes, immortalized MEF cells can be transformed by a single oncogene (Ras) resulting in a loss of contact inhibition, anchorage-independent growth, and tumor formation in nude mice. Studies of MEF cells have played an important role in the elucidation of the molecular mechanisms underlying cellular immortalization, transformation, and tumorigenesis. Additionally, utilization of MEF cells disrupted for specific genes has provided a powerful tool to analyze the genetic regulation of these cellular processes. In this chapter, methods for analysis of cellular immortalization using the 3T3 protocol, as well as transformation of MEF cells using oncogenic retroviruses are provided. This is followed by protocols for analysis of transformed cell characteristics such as foci formation, anchorage independent growth, and tumor formation in nude mice.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
1064-3745
pubmed:author
pubmed:issnType
Print
pubmed:volume
383
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
303-10
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Analysis of transformation and tumorigenicity using mouse embryonic fibroblast cells.
pubmed:affiliation
Brookdale Department of Molecular, Cell and Developmental Biology, Mount Sinai School of Medicine, New York, NY, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural