Linked Life Data

18213600

Source:http://linkedlifedata.com/resource/pubmed/id/18213600

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2008-2-28
pubmed:abstractText
A monolithic capillary column with double mixed-modes of hydrophilic interaction/cation-exchange and RP/cation-exchange stationary phase was prepared by in situ thermal polymerization and then hydrolyzed with hydrochloric acid. The polymerization solution containing glycidyl methacrylate (GMA), 3-sulfopropyl methacrylate potassium salt (SPMA), and ethylene dimethacrylate (EDMA) in a binary porogenic solvent consisting of dimethylformamide (DMF) and 1,4-butanediol was polymerized in a fused-silica capillary pretreated with 3-(trimetoxysilyl) propyl methacrylate. The epoxy groups on the surface were hydrolyzed to diol groups with hydrochloric acid to enhance the polarity of the stationary phase. By simply altering the ACN content in the mobile phase, two mixed-mode mechanisms could be achieved on the same monolithic column in different mobile phase condition. Hydrophilic interaction (or hydrophilic interaction/cation-exchange) was observed at high ACN content, as well as RP (or RP/cation-exchange) was observed at low ACN content. The monolithic column provided good selectivity and high efficiency for separation of neutral polar analytes and basic compounds. Phenols, anilines, alkaloids, nucleic acid bases, and narcotic pharmaceuticals have been successfully separated. Effects of salt concentration and ACN content on the separation have also been investigated. High column efficiencies of up to 352 000 plates/meter were obtained by the separation of narcotic pharmaceuticals.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/1,4-butanediol, http://linkedlifedata.com/resource/pubmed/chemical/3-(trimethoxysilyl)propyl..., http://linkedlifedata.com/resource/pubmed/chemical/Buffers, http://linkedlifedata.com/resource/pubmed/chemical/Butylene Glycols, http://linkedlifedata.com/resource/pubmed/chemical/Cation Exchange Resins, http://linkedlifedata.com/resource/pubmed/chemical/Cations, http://linkedlifedata.com/resource/pubmed/chemical/Dimethylformamide, http://linkedlifedata.com/resource/pubmed/chemical/Epoxy Compounds, http://linkedlifedata.com/resource/pubmed/chemical/Methacrylates, http://linkedlifedata.com/resource/pubmed/chemical/Organosilicon Compounds, http://linkedlifedata.com/resource/pubmed/chemical/Silicon Dioxide, http://linkedlifedata.com/resource/pubmed/chemical/ethylene dimethacrylate, http://linkedlifedata.com/resource/pubmed/chemical/glycidyl methacrylate
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0173-0835
pubmed:author
pubmed:issnType
Print
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
928-35
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Monolithic column with double mixed-modes of hydrophilic interaction/cation-exchange and reverse-phase/cation-exchange stationary phase for pressurized capillary electrochromatography.
pubmed:affiliation
Department of Chemistry, Fuzhou University, Fuzhou, Fujian, P. R. China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't