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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2008-3-25
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pubmed:abstractText |
During immunologic synapse (IS) formation, human CD38 redistributes to the contact area of T cell-antigen-presenting cell (APC) conjugates in an antigen-dependent manner. Confocal microscopy showed that CD38 preferentially accumulated along the contact zone, whereas CD3-zeta redistributed toward the central zone of the IS. APC conjugates with human T cells or B cells transiently expressing CD38-green fluorescent protein revealed the presence of 2 distinct pools of CD38, one localized at the cell membrane and the other in recycling endosomes. Both pools were recruited to the T/APC contact sites and required antigen-pulsed APCs. The process appeared more efficient in T cells than in APCs. CD38 was actively recruited at the IS of T cells by means of Lck-mediated signals. Overexpression of CD38 in T cells increased the levels of antigen-induced intracellular calcium release. Opposite results were obtained by down-regulating surface CD38 expression by means of CD38 siRNA. CD38 blockade in influenza HA-specific T cells inhibited IL-2 and IFN-gamma production, PKC phosphorylation at Thr538, and PKC recruitment to the IS induced by antigen-pulsed APCs. These results reveal a new role for CD38 in modulating antigen-mediated T-cell responses during IS formation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD38,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/CD3 antigen, zeta chain,
http://linkedlifedata.com/resource/pubmed/chemical/CD38 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Hemagglutinin Glycoproteins...,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphocyte Specific Protein...,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0006-4971
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
111
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3653-64
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:18212246-Antigen-Presenting Cells,
pubmed-meshheading:18212246-Antigens, CD3,
pubmed-meshheading:18212246-Antigens, CD38,
pubmed-meshheading:18212246-Antigens, Viral,
pubmed-meshheading:18212246-Calcium Signaling,
pubmed-meshheading:18212246-Down-Regulation,
pubmed-meshheading:18212246-Endosomes,
pubmed-meshheading:18212246-Hemagglutinin Glycoproteins, Influenza Virus,
pubmed-meshheading:18212246-Humans,
pubmed-meshheading:18212246-Immunologic Capping,
pubmed-meshheading:18212246-Jurkat Cells,
pubmed-meshheading:18212246-Lymphocyte Specific Protein Tyrosine Kinase p56(lck),
pubmed-meshheading:18212246-Membrane Glycoproteins,
pubmed-meshheading:18212246-Phosphorylation,
pubmed-meshheading:18212246-Protein Kinase C,
pubmed-meshheading:18212246-RNA, Small Interfering,
pubmed-meshheading:18212246-T-Lymphocytes
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pubmed:year |
2008
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pubmed:articleTitle |
Antigen-induced clustering of surface CD38 and recruitment of intracellular CD38 to the immunologic synapse.
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pubmed:affiliation |
Departamento de Biología Celular e Immunología, Instituto de Parasitología y Biomedicina López-Neyra, Consejo Superior de Investigaciones Científicas, Armilla, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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