Source:http://linkedlifedata.com/resource/pubmed/id/18211966
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
|
pubmed:dateCreated |
2008-3-26
|
pubmed:abstractText |
The manuscript presents definitive studies of surfactant protein D (SP-D) in the context of inflammatory lung fluids. The extent of SP-D depletion in bronchoalveolar lavage fluid (BALF) of children affected with cystic fibrosis (CF) is demonstrated to correlate best with the presence of the active neutrophil serine protease (NSP) elastase. Novel C-terminal SP-D fragments of 27 kDa and 11 kDa were identified in patient lavage fluid in addition to the previously described N-terminal, 35-kDa fragment by the use of isoelectrofocusing, modified blotting conditions, and region-specific antibodies. SP-D cleavage sites were identified. In vitro treatment of recombinant human SP-D dodecamers with NSPs replicated the fragmentation, but unexpectedly, the pattern of SP-D fragments generated by NSPs was dependent on calcium concentration. Whereas the 35- and 11-kDa fragments were generated when incubations were performed in low calcium (200 microM CaCl(2)), incubations in physiological calcium (2 mM) with higher amounts of elastase or proteinase-3 generated C-terminal 27, 21, and 14 kDa fragments, representing cleavage within the collagen and neck regions. Studies in which recombinant SP-D cleavage by individual NSPs was quantitatively evaluated under low and high calcium conditions showed that the most potent NSP for cleaving SP-D is elastase, followed by proteinase-3, followed by cathepsin G. These relative potency findings were considered in the context of other studies that showed that active NSPs in CF BALF are in the order: elastase, followed by cathepsin G, followed by proteinase-3. The findings support a pre-eminent role for neutrophil elastase as the critical protease responsible for SP-D depletion in inflammatory lung disease.
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Apr
|
pubmed:issn |
0741-5400
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
83
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
946-55
|
pubmed:meshHeading |
pubmed-meshheading:18211966-Adolescent,
pubmed-meshheading:18211966-Adult,
pubmed-meshheading:18211966-Asthma,
pubmed-meshheading:18211966-Bronchoalveolar Lavage Fluid,
pubmed-meshheading:18211966-Child,
pubmed-meshheading:18211966-Child, Preschool,
pubmed-meshheading:18211966-Cough,
pubmed-meshheading:18211966-Cystic Fibrosis,
pubmed-meshheading:18211966-Humans,
pubmed-meshheading:18211966-Infant,
pubmed-meshheading:18211966-Leukocyte Elastase,
pubmed-meshheading:18211966-Lung Diseases,
pubmed-meshheading:18211966-Neuromuscular Diseases,
pubmed-meshheading:18211966-Neutrophils,
pubmed-meshheading:18211966-Pulmonary Surfactant-Associated Protein D,
pubmed-meshheading:18211966-Reference Values,
pubmed-meshheading:18211966-Serine Endopeptidases
|
pubmed:year |
2008
|
pubmed:articleTitle |
Patterns of neutrophil serine protease-dependent cleavage of surfactant protein D in inflammatory lung disease.
|
pubmed:affiliation |
Immune Disease Institute, Harvard Medical School, Boston, MA 02115, USA.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|