Source:http://linkedlifedata.com/resource/pubmed/id/18211505
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rdf:type | |
lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0039005,
umls-concept:C0085358,
umls-concept:C0086418,
umls-concept:C0185117,
umls-concept:C0225336,
umls-concept:C1135183,
umls-concept:C1332717,
umls-concept:C1413244,
umls-concept:C1413357,
umls-concept:C1515655,
umls-concept:C1533691,
umls-concept:C1706438,
umls-concept:C1708528,
umls-concept:C2698600,
umls-concept:C2911684
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pubmed:issue |
2
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pubmed:dateCreated |
2008-1-23
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pubmed:abstractText |
Overcoming cell-mediated immunity, especially of human CD8(+) CTLs, is important for the success of xenotransplantation. Our group has previously reported that the cytotoxicity of human CD8(+) CTLs against pig endothelial cells (PEC) is highly detrimental and mediated in major part by the Fas/FasL apoptotic pathway. Cellular FLICE inhibitory protein (c-FLIP) was originally identified as an inhibitor of death-receptor signaling through binding competition with caspase-8 for recruitment to Fas-associated via death domain (FADD). Two major c-FLIP variants result from alternative mRNA splicing: a short, 26-KDa protein (c-FLIP(S)) and a long, 55-KDa form (c-FLIP(L)). The cytoprotective effects of c-FLIP(S/L) in xenograft cells remain controversial. This study demonstrates that the overexpression of c-FLIP(S/L) genes markedly suppress human CD8(+) CTL-mediated xenocytotoxicity and, in addition, the cytoprotective effects of c-FLIP(L) appear to be significantly stronger than those of c-FLIP(S). Furthermore, to prove the prolonged effects of xenograft survival, PEC transfectants with c-FLIP(S/L) genes were transplanted under rat kidney capsules. Prolonged survival was elicited from FLIP(S/L) transfectants, whereas parental PEC was completely rejected through day 5, posttransplant. Thus, intracellular remodeling with the overexpression of c-FLIP(S/L) in xenograft cells may avoid innate cellular attacks against xenografts and facilitate long-term xenograft survival.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1600-6143
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
288-97
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pubmed:meshHeading |
pubmed-meshheading:18211505-Animals,
pubmed-meshheading:18211505-CASP8 and FADD-Like Apoptosis Regulating Protein,
pubmed-meshheading:18211505-Caspases,
pubmed-meshheading:18211505-Cell Line,
pubmed-meshheading:18211505-Cytotoxicity, Immunologic,
pubmed-meshheading:18211505-DNA Fragmentation,
pubmed-meshheading:18211505-Endothelium, Vascular,
pubmed-meshheading:18211505-Genetic Vectors,
pubmed-meshheading:18211505-Humans,
pubmed-meshheading:18211505-Lymphocyte Transfusion,
pubmed-meshheading:18211505-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:18211505-Swine,
pubmed-meshheading:18211505-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:18211505-Transplantation, Heterologous
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pubmed:year |
2008
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pubmed:articleTitle |
In vitro and in vivo prevention of human CD8+ CTL-mediated xenocytotoxicity by pig c-FLIP expression in porcine endothelial cells.
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pubmed:affiliation |
Department of Surgery (E1), Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan. tanemura@surg1.med.osaka-u.ac.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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