Linked Life Data

18211056

Source:http://linkedlifedata.com/resource/pubmed/id/18211056

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2008-2-8
pubmed:abstractText
2-Aminopurine (2AP) is an adenine analogue that has a high fluorescence quantum yield. Its fluorescence yield decreases significantly when the base is incorporated into DNA, making it a very useful real-time probe of DNA structure. However, the basic mechanism underlying 2AP fluorescence quenching by base stacking is not well understood. A critical element in approaching this problem is obtaining an understanding of the electronic structure of the excited state. We have explored the excited state properties of 2AP and 2-amino,9-methylpurine (2A9MP) in frozen solutions using Stark spectroscopy. The experimental data were correlated with high level ab initio (MRCI) calculations of the dipole moments, mu0 and mu1, of the ground and excited states. The magnitude and direction of the dipole moment change, Deltamu01 = mu1 - mu0, of the lowest energy optically allowed transition was determined. While other studies have reported on the magnitude of the dipole moment change, we believe that this is the first report of the direction of Deltamu, a quantity that will be of great value in interpreting absorption spectral changes of the 2AP chromophore. Polarizability changes due to the transition were also obtained.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1520-6106
pubmed:author
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
112
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1789-95
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
2-Aminopurine excited state electronic structure measured by stark spectroscopy.
pubmed:affiliation
Department of Chemistry, Temple University, Philadelphia, PA 19122, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.