Linked Life Data

18209474

Source:http://linkedlifedata.com/resource/pubmed/id/18209474

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-3
pubmed:dateCreated
2008-1-22
pubmed:abstractText
The CFTR protein, encoded by the gene whose mutations induce Cystic Fibrosis, is an anion channel devoted mainly to chloride and bicarbonate transmembrane transport, but which also regulates transport of several other ions. Moreover, it is implicated in the cell response to inflammation, and, reciprocally, cftr gene expression is modulated by inflammatory stimuli and transduction pathways. Looking for a control of CFTR expression by ionic conditions, we investigated the effect of altered extracellular bicarbonate ion concentration on CFTR expression in human pulmonary Calu-3 cells. We found that basal cftr gene transcription is enhanced when extracellular HCO(3)(-) concentration increases from 0 to 25 mmol/l. The transduction pathway controlled by these extracellular [HCO(3)(-)] variations includes cAMP production linked to the stimulation of soluble adenylyl cyclase (sAC), and nuclear accumulation of the transcription factor, CREB. Basal membrane content in CFTR protein exhibits the same variations as cftr mRNA in cells incubated in the presence of extracellular [HCO(3)(-)] between 0 and 25 mmol/l, and is also decreased by inhibiting sAC in the presence of HCO(3)(-). These results show that bicarbonate-controlled sAC stimulation must be taken into account in cell physiology and that basal CFTR expression depends on an ionic parameter.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1015-8987
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
75-86
pubmed:meshHeading
pubmed-meshheading:18209474-Adenylate Cyclase, pubmed-meshheading:18209474-Bicarbonates, pubmed-meshheading:18209474-Cell Line, pubmed-meshheading:18209474-Cell Nucleus, pubmed-meshheading:18209474-Cyclic AMP, pubmed-meshheading:18209474-Cyclic AMP Response Element-Binding Protein, pubmed-meshheading:18209474-Cystic Fibrosis Transmembrane Conductance Regulator, pubmed-meshheading:18209474-Enzyme Activation, pubmed-meshheading:18209474-Gene Expression Regulation, pubmed-meshheading:18209474-Humans, pubmed-meshheading:18209474-Lung, pubmed-meshheading:18209474-Phosphoproteins, pubmed-meshheading:18209474-Phosphorylation, pubmed-meshheading:18209474-Protein Biosynthesis, pubmed-meshheading:18209474-RNA, Messenger, pubmed-meshheading:18209474-Solubility, pubmed-meshheading:18209474-Transcription, Genetic
pubmed:year
2008
pubmed:articleTitle
Control of basal CFTR gene expression by bicarbonate-sensitive adenylyl cyclase in human pulmonary cells.
pubmed:affiliation
Inserm, U845, Paris, F-75015, Université Paris V-Paris Descartes, Faculté de Médecine René Descartes, Paris, F-75075, France. legroscommat@necker.fr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't