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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2008-2-13
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pubmed:abstractText |
RNA interference (RNAi) mediated by small-interfering RNAs (siRNAs) is a highly effective gene-silencing mechanism with great potential for gene-therapeutic applications. siRNA agents also exert non-target-related biological effects and toxicities, including immune-system stimulation. Specifically, siRNA synthesized from the T7 RNA polymerase system triggers a potent induction of type-I interferon (IFN) in a variety of cells. Single-stranded RNA also stimulates innate cytokine responses in mammals. We found that pppGn (n = 2,3) associated with the 5'-end of the short-hairpin RNA (shRNA) from the T7 RNA polymerase system did not induce detectable amounts of IFN. The residual amount of guanine associated with the 5'-end and hairpin structures of the transcript was proportional to the reduction of the IFN response. Here we describe a T7 pppGn (n = 2,3) shRNA synthesis that does not induce the IFN response, and maintains the full efficacy of siRNA.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18208841-10823883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18208841-10938111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18208841-11585785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18208841-12000851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18208841-12655026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18208841-12796781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18208841-12942087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18208841-14681580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18208841-14990954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18208841-15107482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18208841-15152202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18208841-15153468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18208841-16566896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18208841-16648842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18208841-17038589,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18208841-17038590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18208841-1867954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18208841-8197162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18208841-8627772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18208841-8642689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18208841-8794309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18208841-8910525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18208841-8970954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18208841-9094609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18208841-9094610,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18208841-9557686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18208841-9918116
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1362-4962
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
36
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
e18
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:18208841-DNA-Directed RNA Polymerases,
pubmed-meshheading:18208841-Guanine,
pubmed-meshheading:18208841-HIV-1,
pubmed-meshheading:18208841-HeLa Cells,
pubmed-meshheading:18208841-Humans,
pubmed-meshheading:18208841-Interferon Type I,
pubmed-meshheading:18208841-Phosphates,
pubmed-meshheading:18208841-RNA, Untranslated,
pubmed-meshheading:18208841-RNA, Viral,
pubmed-meshheading:18208841-RNA Interference,
pubmed-meshheading:18208841-Viral Proteins,
pubmed-meshheading:18208841-Virus Replication
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pubmed:year |
2008
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pubmed:articleTitle |
Short-hairpin RNAs synthesized by T7 phage polymerase do not induce interferon.
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pubmed:affiliation |
Department of Life and Environmental Science, High Technology Research Center, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino-shi, Chiba 275-0016 Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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