Source:http://linkedlifedata.com/resource/pubmed/id/18204953
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2008-4-14
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| pubmed:databankReference | |
| pubmed:abstractText |
To investigate the potential of double-stranded RNA interferencing with gene expression in Dunaliella salina, a plasmid pBIRNAI-Dsa was constructed to express hairpin RNA (hpRNA) containing sequences homologous to phytoene desaturase gene (pds), a key gene in carotenoid biosynthesis, and transformed into D. salina by electroporation. The relative transcription level of pds in pBIRNAI-Dsa-treated cells to nontreated cells was quantitated and the gene silencing efficiency by RNAi was evaluated via real-time polymerase chain reaction (PCR). The transcriptions of pds of the pBIRNAI-Dsa-treated group changed compared to those of the control group, and the 2(-delta deltaC)(T) was lowest on the 7th day, corresponding to 0.281265-fold of the relative pds control transcript; a relatively strong gene inhibition effect was therefore deduced. The transcript of pds may be modulated in a wide range, and a reduced transcription even to 28% of the normal level may be tolerated for its survival. This study shows that dsRNA-mediated genetic interference can induce sequence-specific inhibition of gene expression and pBIRNAI-Dsa can be used for transient suppression of gene expression in D. salina. The aim of this study was to exploit dsRNA-mediated gene silencing and to provide a foundation for gene function research in D. salina.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:issn |
1436-2228
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
10
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
219-26
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| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:18204953-Base Sequence,
pubmed-meshheading:18204953-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:18204953-Chlorophyta,
pubmed-meshheading:18204953-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:18204953-Molecular Sequence Data,
pubmed-meshheading:18204953-Oxidoreductases,
pubmed-meshheading:18204953-Plasmids,
pubmed-meshheading:18204953-RNA Interference,
pubmed-meshheading:18204953-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:18204953-Time Factors
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| pubmed:articleTitle |
Inhibition of pds gene expression via the RNA interference approach in Dunaliella salina (Chlorophyta).
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| pubmed:affiliation |
Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, People's Republic of China.
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| pubmed:publicationType |
Journal Article
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