Source:http://linkedlifedata.com/resource/pubmed/id/18202799
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2008-1-18
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| pubmed:abstractText |
We previously developed a DNA microarray-based system that out-performs traditionally used clinical parameters for prediction of early intrahepatic recurrence (IHR) of hepatocellular carcinoma (HCC). Because DNA microarray is too expensive for daily clinical use, we used a quantitative real-time reverse transcription-polymerase chain reaction (QRT-PCR) to develop a lower-cost predictor for early IHR. From the 12 early IHR-related genes integrated in the previous predictor, we selected 6 genes whose levels showed the strongest association between data from the 2 distinct DNA microarray platforms with the same sample set. Expression of these 6 genes relative to that of GAPDH was measured by QRT-PCR in 82 HCCs. Of the 82 HCCs, 39 and 43 were assigned to training and independent test sets, respectively. By searching all combinations (n=2-6) of the 6 genes, we found an optimal combination of 3 genes (HLADRA, DDX17 and LAPTM5) that minimized the leave-one-out error for prediction of early IHR in the training set. The 3-gene predictor constructed with the Fisher linear classifier correctly predicted early IHR or non-recurrence in 35 (81.4%) of 43 HCCs in the independent test set and had a high positive predictive value of 72.7% and a high negative predictive value of 84.4%. Multivariate analysis with the stepwise logistic regression showed that the 3-gene predictor [F(x)<0] was an independent risk factor for early IHR (risk ratio, 13.6; p=0.006), indicating its potential as an easy-to-use predictor for accurate prediction of early IHR of HCC.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DDX17 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/DEAD-box RNA Helicases,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DR Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/LAPTM5 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Markers, Biological
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
1021-335X
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| pubmed:author |
pubmed-author:EramotoMasayaM,
pubmed-author:HamaguchiTakashiT,
pubmed-author:HamamotoYoshihikoY,
pubmed-author:IizukaNorioN,
pubmed-author:MiyamotoTakanobuT,
pubmed-author:OkaMasaakiM,
pubmed-author:SakamotoKazuhikoK,
pubmed-author:SawamuraMikikoM,
pubmed-author:SomuraHideakiH,
pubmed-author:TamesaTakaoT,
pubmed-author:TsunedomiRyouichiR,
pubmed-author:Yamada-OkabeHisafumiH
|
| pubmed:issnType |
Print
|
| pubmed:volume |
19
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
489-95
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| pubmed:dateRevised |
2009-11-19
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| pubmed:meshHeading |
pubmed-meshheading:18202799-Aged,
pubmed-meshheading:18202799-Carcinoma, Hepatocellular,
pubmed-meshheading:18202799-DEAD-box RNA Helicases,
pubmed-meshheading:18202799-Early Diagnosis,
pubmed-meshheading:18202799-Female,
pubmed-meshheading:18202799-Gene Expression,
pubmed-meshheading:18202799-Genes, Neoplasm,
pubmed-meshheading:18202799-HLA-DR Antigens,
pubmed-meshheading:18202799-Hepatectomy,
pubmed-meshheading:18202799-Humans,
pubmed-meshheading:18202799-Liver Neoplasms,
pubmed-meshheading:18202799-Male,
pubmed-meshheading:18202799-Membrane Proteins,
pubmed-meshheading:18202799-Middle Aged,
pubmed-meshheading:18202799-Neoplasm Recurrence, Local,
pubmed-meshheading:18202799-Prognosis,
pubmed-meshheading:18202799-RNA, Messenger,
pubmed-meshheading:18202799-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:18202799-Tumor Markers, Biological
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| pubmed:year |
2008
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| pubmed:articleTitle |
A three-gene predictor for early intrahepatic recurrence of hepatocellular carcinoma after curative hepatectomy.
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| pubmed:affiliation |
Department of Surgery II, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|