Linked Life Data

18197735

Source:http://linkedlifedata.com/resource/pubmed/id/18197735

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2008-1-16
pubmed:abstractText
A set of cDNAs of Pelargonium line pattern virus (PLPV) was assembled under the control of T7 RNA polymerase promoter and ligated into the plasmid pUC18. Transcripts synthesized in vitro from cDNA were infectious on Chenopodium quinoa according to locally induced lesions and hybridization assay. The biological activity of the viral transcripts was particularly sensitive to the short 3' terminus extensions, whereas inclusion of the 3 extra bases at the 5' terminus did not substantially affect the infectivity. Inoculation of the transcripts on plants Nicotiana benthamiana and Nicotiana clevelandii give rise to the systemic infection indistinguishable from that established by the parental isolate. This is the first report about the preparation of infectious RNA transcripts from a full-length cDNA clone of PLPV.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0001-723X
pubmed:author
pubmed:issnType
Print
pubmed:volume
51
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
271-4
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Biological activity of transcripts from cDNA of Pelargonium line pattern virus.
pubmed:affiliation
Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas-UPV, Campus Universidad Politécnica de Valencia, Ciudad de la Innovación, Valencia, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't