Source:http://linkedlifedata.com/resource/pubmed/id/18196274
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
2008-3-19
|
| pubmed:abstractText |
Adult bone-marrow-derived mesenchymal stroma cells (BMSC) seem to be a potential cell source for tissue engineering of the ligament. The objective of this work was to study the time-related changes in mRNA expression and protein levels of collagen types I and III and of tenascin-C in BMSC under co-culture with fibroblasts or under a uniaxial cyclic condition. Rat BMSC harvested from the femur and tibial bone marrow were co-cultured with ligament fibroblasts or stimulated by cyclic 10% uniaxial stretching at 1 Hz. Image analysis showed significant cell loss in stretched BMSC, particularly in the directions close to the stretching direction. However, these BMSC displayed an equivalent growth rate to that of non-stretched cells. Real-time reverse transcription/polymerase chain reaction revealed that the mRNA expression of collagen types I and III and of tenascin-C by BMSC was significantly up-regulated by co-culture and cyclic stretching. Radioimmunoassay results confirmed the effects of these stimulations, showing increases in the level of these proteins. Thus, BMSC might be useful as a cell source for the tissue engineering of ligament.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0302-766X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
332
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
101-9
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| pubmed:meshHeading |
pubmed-meshheading:18196274-Animals,
pubmed-meshheading:18196274-Biomechanics,
pubmed-meshheading:18196274-Bone Marrow Cells,
pubmed-meshheading:18196274-Cell Count,
pubmed-meshheading:18196274-Cell Proliferation,
pubmed-meshheading:18196274-Cell Shape,
pubmed-meshheading:18196274-Coculture Techniques,
pubmed-meshheading:18196274-Collagen Type I,
pubmed-meshheading:18196274-Collagen Type III,
pubmed-meshheading:18196274-Fibroblasts,
pubmed-meshheading:18196274-Gene Expression Regulation,
pubmed-meshheading:18196274-Ligaments,
pubmed-meshheading:18196274-Male,
pubmed-meshheading:18196274-Mesenchymal Stem Cells,
pubmed-meshheading:18196274-Rats,
pubmed-meshheading:18196274-Rats, Wistar,
pubmed-meshheading:18196274-Stress, Mechanical,
pubmed-meshheading:18196274-Tenascin,
pubmed-meshheading:18196274-Time Factors
|
| pubmed:year |
2008
|
| pubmed:articleTitle |
Time-related changes in expression of collagen types I and III and of tenascin-C in rat bone mesenchymal stem cells under co-culture with ligament fibroblasts or uniaxial stretching.
|
| pubmed:affiliation |
LEMTA, Nancy University, CNRS, 2 Avenue de la Forêt de Haye, BP 160, 54504 Vandoeuvre-les-Nancy Cedex, France.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|