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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2008-2-4
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pubmed:abstractText |
MicroRNAs (miRNAs) are single-stranded, noncoding RNAs that are important in many biological processes. Although the oncogenic and tumour-suppressive functions of several miRNAs have been characterized, the role of miRNAs in mediating tumour metastasis was addressed only recently and still remains largely unexplored. To identify potential metastasis-promoting miRNAs, we set up a genetic screen using a non-metastatic, human breast tumour cell line that was transduced with a miRNA-expression library and subjected to a trans-well migration assay. We found that human miR-373 and miR-520c stimulated cancer cell migration and invasion in vitro and in vivo, and that certain cancer cell lines depend on endogenous miR-373 activity to migrate efficiently. Mechanistically, the migration phenotype of miR-373 and miR-520c can be explained by suppression of CD44. We found significant upregulation of miR-373 in clinical breast cancer metastasis samples that correlated inversely with CD44 expression. Taken together, our findings indicate that miRNAs are involved in tumour migration and invasion, and implicate miR-373 and miR-520c as metastasis-promoting miRNAs.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1476-4679
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pubmed:author |
pubmed-author:AgamiReuvenR,
pubmed-author:CoukosGeorgeG,
pubmed-author:EganDavid ADA,
pubmed-author:GimottyPhyllis APA,
pubmed-author:GumireddyKiranmaiK,
pubmed-author:HuangGuanghuaG,
pubmed-author:HuangQihongQ,
pubmed-author:KatsarosDionyssiosD,
pubmed-author:Klein-SzantoAndres JAJ,
pubmed-author:LiAnpingA,
pubmed-author:NagelRemcoR,
pubmed-author:NairSureshS,
pubmed-author:PuréEllenE,
pubmed-author:SchrierMarietteM,
pubmed-author:ZhangLinL,
pubmed-author:le SageCarlosC
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pubmed:issnType |
Electronic
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
202-10
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pubmed:dateRevised |
2008-5-21
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pubmed:meshHeading |
pubmed-meshheading:18193036-Animals,
pubmed-meshheading:18193036-Antigens, CD44,
pubmed-meshheading:18193036-Breast Neoplasms,
pubmed-meshheading:18193036-Cell Line, Tumor,
pubmed-meshheading:18193036-Cell Migration Assays,
pubmed-meshheading:18193036-Cell Movement,
pubmed-meshheading:18193036-Colonic Neoplasms,
pubmed-meshheading:18193036-Female,
pubmed-meshheading:18193036-Humans,
pubmed-meshheading:18193036-Lymphatic Metastasis,
pubmed-meshheading:18193036-Male,
pubmed-meshheading:18193036-Mice,
pubmed-meshheading:18193036-Mice, SCID,
pubmed-meshheading:18193036-MicroRNAs,
pubmed-meshheading:18193036-Neoplasm Invasiveness,
pubmed-meshheading:18193036-Neoplasm Metastasis,
pubmed-meshheading:18193036-Neoplasm Transplantation,
pubmed-meshheading:18193036-Prostatic Neoplasms,
pubmed-meshheading:18193036-Transplantation, Heterologous
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pubmed:year |
2008
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pubmed:articleTitle |
The microRNAs miR-373 and miR-520c promote tumour invasion and metastasis.
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pubmed:affiliation |
The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA. qhuang@wistar.org
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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