Linked Life Data

18191892

Source:http://linkedlifedata.com/resource/pubmed/id/18191892

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-3
pubmed:dateCreated
2008-4-25
pubmed:abstractText
The objectives of this study were first to set up and validate the quality criteria of the Xenobiotic Detection Systems-chemical activated luciferase gene expression (CALUX) bioassay for the analysis of cow's milk samples spiked with polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans (PCDD/Fs). The application of this bioassay was then tested by analyzing 28 commercially available pasteurized milk samples and comparing the data with the reference method for PCDD/F analysis. The CALUX criteria from the U.S. EPA and the European Union (EU) were calculated from 16 replaced spiked milk (SM) samples and 8 performance evaluation (PE) samples to validate the CALUX bioassay system. The CALUX bioassay criteria included control chart for quality control (QC) standards, recovery efficiency, and data comparability. The control chart for QC standards were both within the micro+/-2sigma range. The recovery efficiencies ranged from 60.4% to 106% with an average of 79.9% (relative standard deviation (RSD): 20.7%). The mean of data comparability (i.e., relative percent difference, RPD) between CALUX bioassay and high-resolution gas chromatography and high-resolution mass spectrometry (HRGC/HRMS) was 19.0% for SM samples. For the PE samples, Pearson's correlation coefficient between CALUX and the HRGC/HRMS method was 0.953. The high correlation shows that the CALUX system is suitable as a screening method and a semi-quantitative method to analyze the PCDD/F concentration in milk samples. Next, the validated CALUX bioassay was applied to measure 28 commercially available pasteurized milk samples. These milk samples were also analyzed with the HRGC/HRMS method to compare the analysis data from two different methods. There is no false negative sample when applying the bioassay to pasteurized milk and PE samples as a screening method and a semi-quantitative method. The present study indicates that CALUX is a powerful bioassay method for screening a large number of milk samples.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0304-3894
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
154
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1166-72
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Validation of the CALUX bioassay as a screening and semi-quantitative method for PCDD/F levels in cow's milk.
pubmed:affiliation
Department of Environmental Engineering, National Cheng Kung University, Tainan 70101, Taiwan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Validation Studies