Linked Life Data

18189155

Source:http://linkedlifedata.com/resource/pubmed/id/18189155

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2008-2-25
pubmed:abstractText
A new method applying sensitive and selective liquid chromatography coupled with mass spectrometry (LC/MS/MS) for analyzing tertiary-butylhydroquinone (TBHQ) and its metabolites in rat serum was validated. Using an extracted ion chromatogram (EIC) of m/z 149, free TBHQ was observed in rat serum after dosing TBHQ at 350 mg/kg to male and female Sprague-Dawley (SD) rats. Four major metabolites of TBHQ were identified-a TBHQ-sulfate, two TBHQ-sulfate-derived substances and a TBHQ-glucuronide through MS(n) spectra. Besides its simplicity, the sample treatment allows one to obtain a very good recovery of analysts, namely around 95%. This result suggests that the method described here is useful for the analysis of TBHQ and its metabolites in rat serum. Moreover, the metabolism of TBHQ was investigated using the method. After oral administration, TBHQ appear to be more completely absorbed and bio-transformed by males than females, which may result in higher acute oral toxicity of TBHQ for males than females.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0024-4201
pubmed:author
pubmed:issnType
Print
pubmed:volume
43
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
281-8
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Determination of tertiary-butylhydroquinone and its metabolites in rat serum by liquid chromatography-ion trap mass spectrometry.
pubmed:affiliation
Institute for Nanobiomedical Technology and Membrane Biology, Cancer Center, West China Hospital, West China Medical School, Sichuan University, No 1, Chengdu, 610041, China. hwpharm@tom.com
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't