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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2008-3-11
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pubmed:abstractText |
The endoglycosidase EndoS and the cysteine proteinase SpeB from the human pathogen Streptococcus pyogenes are functionally related in that they both hydrolyze IgG leading to impairment of opsonizing antibodies and thus enhance bacterial survival in human blood. In this study, we further investigated the relationship between EndoS and SpeB by examining their in vitro temporal production and stability and activity of EndoS. Furthermore, theoretical structure modeling of EndoS combined with site-directed mutagenesis and chemical blocking of amino acids was used to identify amino acids required for the IgG glycan-hydrolyzing activity of EndoS.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1471-2180
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:18182097-Amino Acid Sequence,
pubmed-meshheading:18182097-Bacterial Proteins,
pubmed-meshheading:18182097-Blotting, Western,
pubmed-meshheading:18182097-Cysteine Endopeptidases,
pubmed-meshheading:18182097-Glutamic Acid,
pubmed-meshheading:18182097-Glycoside Hydrolases,
pubmed-meshheading:18182097-Hydrolysis,
pubmed-meshheading:18182097-Immunoglobulin G,
pubmed-meshheading:18182097-Models, Molecular,
pubmed-meshheading:18182097-Molecular Sequence Data,
pubmed-meshheading:18182097-Mutagenesis, Site-Directed,
pubmed-meshheading:18182097-Polysaccharides,
pubmed-meshheading:18182097-Protein Structure, Secondary,
pubmed-meshheading:18182097-Sequence Homology, Amino Acid,
pubmed-meshheading:18182097-Streptococcus pyogenes,
pubmed-meshheading:18182097-Structural Homology, Protein,
pubmed-meshheading:18182097-Structure-Activity Relationship,
pubmed-meshheading:18182097-Tryptophan
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pubmed:year |
2008
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pubmed:articleTitle |
EndoS from Streptococcus pyogenes is hydrolyzed by the cysteine proteinase SpeB and requires glutamic acid 235 and tryptophans for IgG glycan-hydrolyzing activity.
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pubmed:affiliation |
Department of Clinical Sciences, Division of Infection Medicine, Lund University, Biomedical Center B14, SE-221 84 Lund, Sweden. maria.allhorn@med.lu.se
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pubmed:publicationType |
Journal Article
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