Linked Life Data

18179778

Source:http://linkedlifedata.com/resource/pubmed/id/18179778

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2008-3-5
pubmed:abstractText
Methyl jasmonate (MeJA) and cerium (Ce(4+)) elicitation share common features of increasing taxol accumulation of Taxus cuspidata cells. Interestingly, Ce(4+) induces programmed cell death (PCD), but this phenomenon is not observed with MeJA elicitation. Here, using a lipidomic approach to measure more than 100 membrane glycerophospholipids of T. cuspidata cells quantitatively, we discovered that lysophosphatidylcholine (LysoPC), phosphatidic acid (PA) and phosphatidylcholine were three potential lipid markers that were responsible for the differences between Ce(4+)-induced cells and MeJA-induced cells. Compared with MeJA elicitation, marked increase of phospholipase D (PLD) activity was observed following Ce(4+) elicitation, suggesting that the PLD activation and high concentrations of PA production might mediate the PCD. Rapid increase of phospholipase A(2) (PLA(2)) activity caused the release of fatty acids and LysoPC following Ce(4+) elicitation, which enhanced endogenous jasmonic acid (JA) accumulation. In contrast, PLA(2) activity was poorly induced following MeJA elicitation. PLA(2) inhibitor suppressed not only JA accumulation but also taxol production, suggesting that the PLA(2) activation mediated Ce(4+)-induced taxol production partially through a JA-dependent signaling pathway. These results demonstrate that differential alternation of glycerolphospholipids caused by phospholipases constitutes an important step in cell death response to Ce(4+) and increasing taxol production.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:volume
1781
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
123-34
pubmed:meshHeading
pubmed-meshheading:18179778-Acetic Acids, pubmed-meshheading:18179778-Apoptosis, pubmed-meshheading:18179778-Cell Membrane, pubmed-meshheading:18179778-Cerium, pubmed-meshheading:18179778-Chromatography, Liquid, pubmed-meshheading:18179778-Cyclopentanes, pubmed-meshheading:18179778-Enzyme Activation, pubmed-meshheading:18179778-Enzyme Inhibitors, pubmed-meshheading:18179778-Glycerophospholipids, pubmed-meshheading:18179778-Lipid Metabolism, pubmed-meshheading:18179778-Models, Biological, pubmed-meshheading:18179778-Multivariate Analysis, pubmed-meshheading:18179778-Oxylipins, pubmed-meshheading:18179778-Paclitaxel, pubmed-meshheading:18179778-Phospholipase D, pubmed-meshheading:18179778-Phospholipases A2, pubmed-meshheading:18179778-Spectrometry, Mass, Electrospray Ionization, pubmed-meshheading:18179778-Taxus
pubmed:year
2008
pubmed:articleTitle
Lipidomic analysis reveals differential defense responses of Taxus cuspidata cells to two elicitors, methyl jasmonate and cerium (Ce4+).
pubmed:affiliation
Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't