Linked Life Data

18178216

Source:http://linkedlifedata.com/resource/pubmed/id/18178216

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2008-1-29
pubmed:abstractText
Congenic mouse strains bearing allelic variants of CD45 are often used in adoptive transfer experiments. Here, we report that immune CD8(+) splenocytes of donor origin acquired the recipient's congenic CD45 marker during interaction with antigen-bearing cells, presumably as a result of membrane transfer upon dissolution of the immunological synapse. Acquisition of recipient marker by donor cells was most prominent after in vitro incubation with peptide for intracellular cytokine staining, where most of the antigen-bearing splenocytes are of recipient origin. In consequence, when antibodies against the recipient's congenic marker were used to distinguish donor and recipient populations, donor origin cells were incorrectly interpreted as being of recipient origin. This phenomenon may cause problems for interpretation of data in adoptive transfer experiments primarily when (a) staining for the recipient's congenic marker and (b) identifying antigen-specific populations by staining for intracellular cytokine.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:day
31
pubmed:volume
330
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
137-45
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
T cell acquisition of APC membrane can impact interpretation of adoptive transfer experiments using CD45 congenic mouse strains.
pubmed:affiliation
Oregon Health and Science University, Molecular Microbiology and Immunology, Portland, OR 97239, USA.
pubmed:publicationType
Journal Article