Source:http://linkedlifedata.com/resource/pubmed/id/18177638
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2008-2-11
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pubmed:abstractText |
Cell spreading and exploration of topographically complex substrates require tightly-regulated interactions between extracellular matrix receptors and the cytoskeleton, but the molecular determinants of these interactions are not defined. We examined whether the actin-binding proteins cortactin, vinculin and filamin A are involved in the formation of the earliest extensions of cells spreading over collagen or poly-L-lysine-coated smooth and beaded substrates. Spreading of human gingival fibroblasts was substantially reduced on beaded or poly-L-lysine-coated substrates. Filamin A, vinculin and cortactin were found in cell extensions on smooth collagen. HEK-293 cells also spread rapidly on smooth collagen and formed numerous cell extensions enriched with filamin A. Knockdown of filamin A in HEK-293 cells by short hairpin RNA reduced spreading and the number of cell extensions. Blocking beta1 integrin function significantly reduced cell spreading and localization of filamin A to cell extensions. Conversely, filamin A-knockdown reduced beta1 integrin-collagen binding as measured by 12G10 antibody, suggesting co-dependence between filamin A and beta1 integrin functions. TUNEL staining showed higher percentages of apoptosis after filamin A-knockdown in spreading cells. Chelation of [Ca2+]i with BAPTA/AM reduced spreading of wild-type and filamin A-knockdown cells, however wild-type cells showed recruitment of filamin A to the subcortex, indicating independent roles of filamin A and [Ca2+]i in cell spreading. We conclude that filamin A integrates with beta1 integrins to mediate cell spreading and prevent apoptosis.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD29,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen,
http://linkedlifedata.com/resource/pubmed/chemical/Contractile Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Microfilament Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/filamins
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0014-4827
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
314
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
834-46
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pubmed:meshHeading |
pubmed-meshheading:18177638-Antigens, CD29,
pubmed-meshheading:18177638-Calcium,
pubmed-meshheading:18177638-Cell Adhesion,
pubmed-meshheading:18177638-Cell Line,
pubmed-meshheading:18177638-Cell Surface Extensions,
pubmed-meshheading:18177638-Cell Survival,
pubmed-meshheading:18177638-Collagen,
pubmed-meshheading:18177638-Contractile Proteins,
pubmed-meshheading:18177638-Fibroblasts,
pubmed-meshheading:18177638-Gingiva,
pubmed-meshheading:18177638-Humans,
pubmed-meshheading:18177638-Microfilament Proteins
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pubmed:year |
2008
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pubmed:articleTitle |
Filamin A regulates cell spreading and survival via beta1 integrins.
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pubmed:affiliation |
CIHR Group in Matrix Dynamics, University of Toronto, Toronto, Canada. hugh.kim@utoronto.ca
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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