| pubmed-article:18175198 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18175198 | lifeskim:mentions | umls-concept:C1138601 | lld:lifeskim |
| pubmed-article:18175198 | lifeskim:mentions | umls-concept:C1556094 | lld:lifeskim |
| pubmed-article:18175198 | lifeskim:mentions | umls-concept:C0205147 | lld:lifeskim |
| pubmed-article:18175198 | lifeskim:mentions | umls-concept:C0596988 | lld:lifeskim |
| pubmed-article:18175198 | lifeskim:mentions | umls-concept:C1442161 | lld:lifeskim |
| pubmed-article:18175198 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:18175198 | pubmed:dateCreated | 2008-2-12 | lld:pubmed |
| pubmed-article:18175198 | pubmed:abstractText | Japanese pear (Pyrus pyrifolia Nakai) has a gametophytic self-incompatibility (GSI) mechanism controlled by a single S-locus with multiple S-haplotypes, each of which contains separate genes that determine the allelic identity of pistil and pollen. The pistil S gene is the S-ribonuclease (S-RNase) gene, whereas good candidates for the pollen S gene are the F-box protein genes. A self-compatible (SC) cultivar, 'Osa-Nijisseiki', which is a bud mutant of 'Nijisseiki' (S (2) S (4)), has a stylar-part mutant S(4)sm-haplotype, which lacks the S (4)-RNase gene but retains the pollen S gene. To delineate the deletion breakpoint in the S(4)sm-haplotype, we constructed a bacterial artificial chromosome (BAC) library from an S (4)-homozygote, and assembled a BAC contig of 570 kb around the S (4)-RNase. Genomic PCR of DNA from S (4)- and S(4)sm-homozygotes and the DNA sequence of the BAC contig allowed the identification of a deletion of 236 kb spanning from 48 kb upstream to 188 kb downstream of S (4)-RNase. The S(4)sm-haplotype lacks 34 predicted open reading frames (ORFs) including the S (4)-RNase and a pollen-specific F-box protein gene (termed as S (4) F-box0). Genomic PCR with a primer pair designed from the deletion junctions yielded a product specific for the S(4)sm-haplotype. The product could be useful as a maker for early selection of SC cultivars harboring the S(4)sm-haplotype. | lld:pubmed |
| pubmed-article:18175198 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18175198 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18175198 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18175198 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18175198 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18175198 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18175198 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18175198 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:18175198 | pubmed:issn | 0167-4412 | lld:pubmed |
| pubmed-article:18175198 | pubmed:author | pubmed-author:NakanishiTets... | lld:pubmed |
| pubmed-article:18175198 | pubmed:author | pubmed-author:SawamuraYutak... | lld:pubmed |
| pubmed-article:18175198 | pubmed:author | pubmed-author:MatsumotoTats... | lld:pubmed |
| pubmed-article:18175198 | pubmed:author | pubmed-author:MoriyaYukiY | lld:pubmed |
| pubmed-article:18175198 | pubmed:author | pubmed-author:OkadaKazumaK | lld:pubmed |
| pubmed-article:18175198 | pubmed:author | pubmed-author:NoriokaNaokoN | lld:pubmed |
| pubmed-article:18175198 | pubmed:author | pubmed-author:TonakaNozomiN | lld:pubmed |
| pubmed-article:18175198 | pubmed:author | pubmed-author:Takasaki-Yasu... | lld:pubmed |
| pubmed-article:18175198 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:18175198 | pubmed:volume | 66 | lld:pubmed |
| pubmed-article:18175198 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18175198 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18175198 | pubmed:pagination | 389-400 | lld:pubmed |
| pubmed-article:18175198 | pubmed:dateRevised | 2011-6-17 | lld:pubmed |
| pubmed-article:18175198 | pubmed:meshHeading | pubmed-meshheading:18175198... | lld:pubmed |
| pubmed-article:18175198 | pubmed:meshHeading | pubmed-meshheading:18175198... | lld:pubmed |
| pubmed-article:18175198 | pubmed:meshHeading | pubmed-meshheading:18175198... | lld:pubmed |
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| pubmed-article:18175198 | pubmed:meshHeading | pubmed-meshheading:18175198... | lld:pubmed |
| pubmed-article:18175198 | pubmed:meshHeading | pubmed-meshheading:18175198... | lld:pubmed |
| pubmed-article:18175198 | pubmed:meshHeading | pubmed-meshheading:18175198... | lld:pubmed |
| pubmed-article:18175198 | pubmed:meshHeading | pubmed-meshheading:18175198... | lld:pubmed |
| pubmed-article:18175198 | pubmed:meshHeading | pubmed-meshheading:18175198... | lld:pubmed |
| pubmed-article:18175198 | pubmed:meshHeading | pubmed-meshheading:18175198... | lld:pubmed |
| pubmed-article:18175198 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18175198 | pubmed:articleTitle | Deletion of a 236 kb region around S 4-RNase in a stylar-part mutant S 4sm-haplotype of Japanese pear. | lld:pubmed |
| pubmed-article:18175198 | pubmed:affiliation | Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo 657-8501, Japan. | lld:pubmed |
| pubmed-article:18175198 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18175198 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:18175198 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:18175198 | lld:pubmed |
