Linked Life Data

18175198

Source:http://linkedlifedata.com/resource/pubmed/id/18175198

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2008-2-12
pubmed:abstractText
Japanese pear (Pyrus pyrifolia Nakai) has a gametophytic self-incompatibility (GSI) mechanism controlled by a single S-locus with multiple S-haplotypes, each of which contains separate genes that determine the allelic identity of pistil and pollen. The pistil S gene is the S-ribonuclease (S-RNase) gene, whereas good candidates for the pollen S gene are the F-box protein genes. A self-compatible (SC) cultivar, 'Osa-Nijisseiki', which is a bud mutant of 'Nijisseiki' (S (2) S (4)), has a stylar-part mutant S(4)sm-haplotype, which lacks the S (4)-RNase gene but retains the pollen S gene. To delineate the deletion breakpoint in the S(4)sm-haplotype, we constructed a bacterial artificial chromosome (BAC) library from an S (4)-homozygote, and assembled a BAC contig of 570 kb around the S (4)-RNase. Genomic PCR of DNA from S (4)- and S(4)sm-homozygotes and the DNA sequence of the BAC contig allowed the identification of a deletion of 236 kb spanning from 48 kb upstream to 188 kb downstream of S (4)-RNase. The S(4)sm-haplotype lacks 34 predicted open reading frames (ORFs) including the S (4)-RNase and a pollen-specific F-box protein gene (termed as S (4) F-box0). Genomic PCR with a primer pair designed from the deletion junctions yielded a product specific for the S(4)sm-haplotype. The product could be useful as a maker for early selection of SC cultivars harboring the S(4)sm-haplotype.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0167-4412
pubmed:author
pubmed:issnType
Print
pubmed:volume
66
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
389-400
pubmed:dateRevised
2011-6-17
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Deletion of a 236 kb region around S 4-RNase in a stylar-part mutant S 4sm-haplotype of Japanese pear.
pubmed:affiliation
Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo 657-8501, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't