Linked Life Data

18174394

Source:http://linkedlifedata.com/resource/pubmed/id/18174394

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2008-4-3
pubmed:abstractText
We studied the effect of hyperbaric oxygen (HBO) preconditioning on the molecular mechanisms of neuroprotection in a rat focal cerebral ischemic model. Seventy-two male Sprague-Dawley rats were pretreated with HBO (100% O(2), 2 atmospheres absolute, 1 h once every other day for 5 sessions) or with room air. In experiment 1, HBO-preconditioned rats and matched room air controls were subjected to focal cerebral ischemia or sham surgery. Postinjury motor parameters and infarction volumes of HBO-preconditioned rats were compared with those of controls. In experiment 2, HBO-preconditioned rats and matched room air controls were killed at different time points. Brain levels of hypoxia-inducible factor-1alpha (HIF-1alpha) and its downstream target gene erythropoietin (EPO) analyzed by Western blotting and RT-PCR as well as HIF-1alpha DNA-binding and transcriptional activities were determined in the ipsilateral hemisphere. HBO induced a marked increase in the protein expressions of HIF-1alpha and EPO and the activity of HIF-1alpha, as well as the expression of EPO mRNA. HBO preconditioning dramatically improved the neurobehavioral outcome at all time points (3.0 +/- 2.1 vs. 5.6 +/- 1.5 at 4 h, 5.0 +/- 1.8 vs. 8.8 +/- 1.4 at 8 h, 6.4 +/- 1.8 vs. 9.7 +/- 1.3 at 24 h; P < 0.01, respectively) and reduced infarction volumes (20.7 +/- 4.5 vs. 12.5 +/- 3.6%, 2,3,5-Triphenyltetrazolium chloride staining) after cerebral ischemia. This observation indicates that the neuroprotection induced by HBO preconditioning may be mediated by an upregulation of HIF-1alpha and its target gene EPO.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
8750-7587
pubmed:author
pubmed:issnType
Print
pubmed:volume
104
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1185-91
pubmed:meshHeading
pubmed-meshheading:18174394-Animals, pubmed-meshheading:18174394-Behavior, Animal, pubmed-meshheading:18174394-Blotting, Western, pubmed-meshheading:18174394-Brain Ischemia, pubmed-meshheading:18174394-Cerebral Infarction, pubmed-meshheading:18174394-Cerebrovascular Circulation, pubmed-meshheading:18174394-DNA, pubmed-meshheading:18174394-Erythropoietin, pubmed-meshheading:18174394-Forelimb, pubmed-meshheading:18174394-Hyperbaric Oxygenation, pubmed-meshheading:18174394-Hypoxia-Inducible Factor 1, alpha Subunit, pubmed-meshheading:18174394-Ischemic Preconditioning, pubmed-meshheading:18174394-Male, pubmed-meshheading:18174394-Neuroprotective Agents, pubmed-meshheading:18174394-Oxygen, pubmed-meshheading:18174394-RNA, Messenger, pubmed-meshheading:18174394-Rats, pubmed-meshheading:18174394-Rats, Sprague-Dawley, pubmed-meshheading:18174394-Reperfusion Injury, pubmed-meshheading:18174394-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:18174394-Tetrazolium Salts, pubmed-meshheading:18174394-Up-Regulation
pubmed:year
2008
pubmed:articleTitle
Mechanism of ischemic tolerance induced by hyperbaric oxygen preconditioning involves upregulation of hypoxia-inducible factor-1alpha and erythropoietin in rats.
pubmed:affiliation
Department of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, People's Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't