Linked Life Data

18165855

Source:http://linkedlifedata.com/resource/pubmed/id/18165855

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2008-2-26
pubmed:abstractText
A method is described whereby the concentrations and 2H isotope enrichment of glutathione (GSH) and cysteine can be simultaneously determined in a single gas chromatography-mass spectrometry run following derivatization as their N,S-ethoxycarbonyl methyl esters. Improvements of the derivatization protocol and the use of a short gas chromatography column combined with single-ion monitoring allow for rapid quantification of these parameters with acceptable precision and reproducibility (coefficient of variation less than 5%). The method makes possible their quantitative measurement in very small volumes (50 microL) of human umbilical cord blood, and is thus suitable for determining the cysteine and GSH concentrations and 2H isotope enrichments in blood samples from neonates or in other conditions in which sample size is restricted. It is shown that the fractional synthesis rate of human umbilical erythrocyte lysates in vitro is several-fold greater than that measured in vivo.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1618-2650
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
390
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1403-12
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Simultaneous determination of glutathione and cysteine concentrations and 2H enrichments in microvolumes of neonatal blood using gas chromatography-mass spectrometry.
pubmed:affiliation
INRA, UMR Physiologie des Adaptations Nutritionnelles, and Université de Nantes, CHU de Nantes, Hotel-Dieu HNB-1, 44093, Nantes Cedex 1, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't