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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2008-1-9
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pubmed:databankReference |
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pubmed:abstractText |
Antibodies that bind protein antigens are indispensable in biochemical research and modern medicine. However, knowledge of RNA-binding antibodies and their application in the ever-growing RNA field is lacking. Here we have developed a robust approach using a synthetic phage-display library to select specific antigen-binding fragments (Fabs) targeting a large functional RNA. We have solved the crystal structure of the first Fab-RNA complex at 1.95 A. Capability in phasing and crystal contact formation suggests that the Fab provides a potentially valuable crystal chaperone for RNA. The crystal structure reveals that the Fab achieves specific RNA binding on a shallow surface with complementarity-determining region (CDR) sequence diversity, length variability, and main-chain conformational plasticity. The Fab-RNA interface also differs significantly from Fab-protein interfaces in amino acid composition and light-chain participation. These findings yield valuable insights for engineering of Fabs as RNA-binding modules and facilitate further development of Fabs as possible therapeutic drugs and biochemical tools to explore RNA biology.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-10582166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-10623545,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-11075354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-11298439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-11500966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-11980483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-12110898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-12163074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-12202384,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-15175762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-15236968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-15261575,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-15525509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-15539566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-15854651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-16121397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-16141072,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-16153177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-16413576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-16424916,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-16604082,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-16622479,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-2258934,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-7523684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-7684607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-8095303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-8341699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-8497480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-8552589,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-8552677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-8743315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-8781224,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/18162543-9783582
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1091-6490
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
8
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pubmed:volume |
105
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
82-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:18162543-Animals,
pubmed-meshheading:18162543-Antibodies,
pubmed-meshheading:18162543-Antigens,
pubmed-meshheading:18162543-Magnesium,
pubmed-meshheading:18162543-Crystallization,
pubmed-meshheading:18162543-Tetrahymena,
pubmed-meshheading:18162543-Kinetics,
pubmed-meshheading:18162543-Biochemistry,
pubmed-meshheading:18162543-RNA,
pubmed-meshheading:18162543-Crystallography, X-Ray,
pubmed-meshheading:18162543-Base Sequence,
pubmed-meshheading:18162543-Amino Acid Sequence,
pubmed-meshheading:18162543-Molecular Sequence Data,
pubmed-meshheading:18162543-Nucleic Acid Conformation,
pubmed-meshheading:18162543-Molecular Conformation,
pubmed-meshheading:18162543-Sequence Homology, Amino Acid,
pubmed-meshheading:18162543-Computational Biology
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