Source:http://linkedlifedata.com/resource/pubmed/id/18160848
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
2007-12-27
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pubmed:abstractText |
HIV-1 Tat is considered to be one of key players to facilitate monocyte entry into the CNS, which is characteristic feature of AIDS-related encephalitis and dementia. This study was performed to determine the regulatory function of superoxide dismutase (SOD) on the HIV-1 Tat-induced signaling pathways leading to NF-kappaB activation, expression of adhesion molecules, and monocyte adhesion in CRT-MG human astroglioma cells by using cell-permeable SOD. When cell-permeable SOD was added to the culture medium of CRT-MG cells, it rapidly entered the cells in dose- and time-dependent manners. Treatment of astrocytes with cell-permeable SOD led to decrease in Tat-induced ROS generation as well as NF-kappaB activation. Cell-permeable SOD inhibited the activation of MAP kinases including ERK, JNK and p38 by HIV-1 Tat. Treatment of CRT-MG cells with cell-permeable SOD significantly inhibited protein and mRNA levels of ICAM-1 and VCAM-1 up-regulated by HIV-1 Tat, as measured by Western blot analysis and RT-PCR. Furthermore, enhanced adhesiveness of monocyte to astrocyte by HIV-1 Tat was significantly abrogated by pretreatment with cell-permeable SOD fusion proteins. These data indicate that SOD has a regulatory function for HIV-1 Tat-induced NF-kappaB activation in astrocytes and suggest that cell-permeable SOD can be used as a feasible therapeutic agent for regulation of ROS-related neurological diseases.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1226-3613
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
39
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
778-86
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pubmed:meshHeading |
pubmed-meshheading:18160848-Astrocytes,
pubmed-meshheading:18160848-Cell Adhesion,
pubmed-meshheading:18160848-Cell Membrane Permeability,
pubmed-meshheading:18160848-Gene Products, tat,
pubmed-meshheading:18160848-HIV Infections,
pubmed-meshheading:18160848-HIV-1,
pubmed-meshheading:18160848-Humans,
pubmed-meshheading:18160848-Monocytes,
pubmed-meshheading:18160848-Signal Transduction,
pubmed-meshheading:18160848-Superoxide Dismutase
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pubmed:year |
2007
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pubmed:articleTitle |
Suppression of HIV-1 Tat-induced monocyte adhesiveness by a cell-permeable superoxide dismutase in astrocytes.
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pubmed:affiliation |
Department of Biomedical Science, College of Natural Sciences, Hallym University, Chuncheon 200-702, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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