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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
|
pubmed:dateCreated |
1992-6-10
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pubmed:abstractText |
The adherence of lipopolysaccharides (LPSs) from periodontal disease-associated bacteria to saliva-coated hydroxyapatite (S-HA) and serum-coated HA beads was examined by chromogenic Limulus activity (toxicolor test). Phenol-water extracted LPS preparations from Bacteroides intermedius, Fusobacterium nucleatum, Actinobacillus actinomycetemcomitans, Eikenella corrodens, and rough-type LPS from Escherichia coli adhered to S-HA and serum-coated beads and agglutinated human erythrocytes. The adhered LPSs to S-HA and serum-coated HA beads were not removed by vigorous washing with distilled water. LPSs from Bacteroides (Porphyromonas) gingivalis strains and wild-type E. coli did not adhere to S-HA, serum-coated HA beads or show hemagglutinating activity. SDS-PAGE patterns stained with silver stain showed that LPSs adhered to S-HA, and serum-coated HA beads and erythrocytes possessed a distinct fast-migrating band similar to rough-type LPS. B. gingivalis LPSs possessed slow-migrating and repeating ladder bands similar to wild-type LPS.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
D
|
pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0902-0055
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
6
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pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
241-5
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1812466-Bacterial Adhesion,
pubmed-meshheading:1812466-Blood,
pubmed-meshheading:1812466-Dental Pellicle,
pubmed-meshheading:1812466-Dental Plaque,
pubmed-meshheading:1812466-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1812466-Gram-Negative Bacteria,
pubmed-meshheading:1812466-Hemagglutination,
pubmed-meshheading:1812466-Hydroxyapatites,
pubmed-meshheading:1812466-Lipopolysaccharides,
pubmed-meshheading:1812466-Saliva,
pubmed-meshheading:1812466-Surface Properties
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pubmed:year |
1991
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pubmed:articleTitle |
Adherence to experimental pellicle of rough-type lipopolysaccharides from subgingival plaque bacteria.
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pubmed:affiliation |
Department of Microbiology, Tokyo Dental College, Chiba City.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|