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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1992-6-2
|
| pubmed:abstractText |
Yeast artificial chromosome (YAC) cloning vectors allow the isolation and analysis of very large segments of DNA. Barley DNA was cleaved with the rare-cutting restriction enzyme MluI and fractionated according to size on a linear sucrose gradient or by pulsed field gel electrophoresis. DNA fragments of approximately 50-250 kb were ligated with the YAC vector pYAC-RC and transformed into yeast spheroplasts. The presence of recombinant YACs with barley DNA inserts was established, and a number of clones containing copies of the repeated element BIS-1 were isolated. These results show that YAC cloning techniques can be successfully applied to the analysis of the barley genome.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0018-0661
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
115
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
133-8
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading | |
| pubmed:year |
1991
|
| pubmed:articleTitle |
Construction of yeast artificial chromosomes containing barley DNA and the identification of clones carrying copies of the repeated element BIS-1.
|
| pubmed:affiliation |
Department of Physiology, Carlsberg Laboratory, Copenhagen Valby, Denmark.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|