Source:http://linkedlifedata.com/resource/pubmed/id/18097044
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2007-12-21
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pubmed:abstractText |
Evidence obtained from both animal models and humans suggests that T cells specific for HSV-1 and HSV-2 glycoprotein D (gD) contribute to protective immunity against herpes infection. However, knowledge of gD-specific human T cell responses is limited to CD4+ T cell epitopes, with no CD8+ T cell epitopes identified to date. In this study, we screened the HSV-1 gD amino acid sequence for HLA-A*0201-restricted epitopes using several predictive computational algorithms and identified 10 high probability CD8+ T cell epitopes. Synthetic peptides corresponding to four of these epitopes, each nine to 10 amino acids in length, exhibited high-affinity binding in vitro to purified human HLA-A*0201 molecules. Three of these four peptide epitopes, gD53-61, gD70-78, and gD278-286, significantly stabilized HLA-A*0201 molecules on T2 cell lines and are highly conserved among and between HSV-1 and HSV-2 strains. Consistent with this, in 33 sequentially studied HLA-A*0201-positive, HSV-1-seropositive, and/or HSV-2-seropositive healthy individuals, the most frequent and robust CD8+ T cell responses, assessed by IFN-gamma ELISPOT, CD107a/b cytotoxic degranulation, and tetramer assays, were directed mainly against gD53-61, gD70-78, and gD278-286 epitopes. In addition, CD8+ T cell lines generated by gD53-61, gD70-78, and gD278-286 peptides recognized infected target cells expressing native gD. Lastly, CD8+ T cell responses specific to gD53-61, gD70-78, and gD278-286 epitopes were induced in HLA-A*0201 transgenic mice following ocular or genital infection with either HSV-1 or HSV-2. The functional gD CD8+ T cell epitopes described herein are potentially important components of clinical immunotherapeutic and immunoprophylactic herpes vaccines.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD8,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes, T-Lymphocyte,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-A*02:01 antigen,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-A Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-A2 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Herpesvirus Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Envelope Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/glycoprotein D, Human herpesvirus 1,
http://linkedlifedata.com/resource/pubmed/chemical/glycoprotein D-herpes simplex...,
http://linkedlifedata.com/resource/pubmed/chemical/herpes simplex virus-1...
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-1767
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pubmed:author |
pubmed-author:BenMohamedLbachirL,
pubmed-author:BettahiIlhamI,
pubmed-author:BuusSørenS,
pubmed-author:ChentoufiAziz AlamiAA,
pubmed-author:DasguptaGargiG,
pubmed-author:LamberthKasperK,
pubmed-author:MohebbiAmirA,
pubmed-author:NesburnAnthony BAB,
pubmed-author:NguyenAlexA,
pubmed-author:WechslerSteven LSL,
pubmed-author:WuMichelleM,
pubmed-author:ZhangXiuliX,
pubmed-author:ZhuXiaomingX
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
180
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
426-37
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:18097044-Animals,
pubmed-meshheading:18097044-Antigens, CD8,
pubmed-meshheading:18097044-Computational Biology,
pubmed-meshheading:18097044-Cytotoxicity, Immunologic,
pubmed-meshheading:18097044-Epitope Mapping,
pubmed-meshheading:18097044-Epitopes, T-Lymphocyte,
pubmed-meshheading:18097044-Female,
pubmed-meshheading:18097044-HLA-A Antigens,
pubmed-meshheading:18097044-HLA-A2 Antigen,
pubmed-meshheading:18097044-Herpesvirus Vaccines,
pubmed-meshheading:18097044-Humans,
pubmed-meshheading:18097044-Male,
pubmed-meshheading:18097044-Mice,
pubmed-meshheading:18097044-Mice, Transgenic,
pubmed-meshheading:18097044-Peptides,
pubmed-meshheading:18097044-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:18097044-Viral Envelope Proteins
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pubmed:year |
2008
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pubmed:articleTitle |
HLA-A*0201-restricted CD8+ cytotoxic T lymphocyte epitopes identified from herpes simplex virus glycoprotein D.
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pubmed:affiliation |
Laboratory of Cellular and Molecular Immunology, Eye Institute, University of California Irvine, School of Medicine, Irvine, CA 92697, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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