Linked Life Data

18093667

Source:http://linkedlifedata.com/resource/pubmed/id/18093667

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2008-2-22
pubmed:abstractText
Caprine herpesvirus 1 (CpHV-1) is an alphaherpesvirus interfering with goat reproductive performances. The virus is associated with neonatal mortality in kids and reproductive failure in adults. A real-time PCR assay based on TaqMan technology and targeting the gene encoding for glycoprotein C (gC) was developed for detection and quantitation of CpHV-1 in samples collected from infected goats. The detection limit of the assay was 1 x 10(2) standard DNA copies, with a sensitivity of 1-2 logs higher than the conventional gel-based PCR assay targeting the same gene. The real-time PCR was reproducible, as shown by satisfactory low intra-assay and interassay coefficients of variation. The quantitative assay was validated on clinical samples, including genital swabs and various tissue samples collected from goats either infected naturally or experimentally with CpHV-1. The high sensitivity, simplicity and reproducibility of the CpHV-1 fluorogenic PCR assay, combined with its wide dynamic range and high throughput, make this method especially suitable for studies on the pathogenesis and for trials with experimental vaccines and antiviral drugs.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0166-0934
pubmed:author
pubmed:issnType
Print
pubmed:volume
148
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
155-60
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Development of a real-time PCR for the detection and quantitation of caprine herpesvirus 1 in goats.
pubmed:affiliation
Department of Animal Health and Well-being, Faculty of Veterinary Medicine, University of Bari, Sp Casamassima km. 3, 70010 Valenzano, Italy.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't, Evaluation Studies