Linked Life Data

18093002

Source:http://linkedlifedata.com/resource/pubmed/id/18093002

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2007-12-20
pubmed:abstractText
We observed Anopheles gambiae sensu stricto stocks that contained both Mopti (M) and Savanna (S) rDNA intergenic spacers (IGS). ASEMBO1 male IGS sequences consistently had a mixture. A diagnostic M and S Hha I restriction enzyme site in these fragments was concordant with two SNPs associated with M and S. Standard M and S stocks demonstrated X-chromosome-only inheritance of the rDNA form, but the ASEMBO1 males showed X and Y chromosome linkage of mixed rDNA. The metaphase Y chromosomes of ASEMBO1 contained a significantly larger amount of DNA relative to the X than a standard S stock. Analysis of wild A. gambiae males from the putative location of origin of the ASEMBO1 stock did not detect the same pattern of polymorphism observed in the laboratory stock but did detect heterogeneous arrays including some missing a diagnostic Hha I restriction site. These results demonstrate that M and S IGS types can occur within the rDNA arrays of a chromatid in laboratory A. gambiae stocks, and some A. gambiae s.s. have rDNA on the Y chromosome.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0962-1075
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
735-41
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
X and Y chromosome inheritance and mixtures of rDNA intergenic spacer regions in Anopheles gambiae.
pubmed:affiliation
Centers for Disease Control and Prevention (CDC) and Atlanta Research and Education Foundation, Atlanta, GA, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, N.I.H., Extramural